问题解决测试：用限制性核酸内切酶消化质粒

Problem-solving test: digestion of a plasmid with restriction endonucleases.

作者信息

Szeberényi József

机构信息

Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary.

出版信息

Biochem Mol Biol Educ. 2013 May-Jun;41(3):189-90. doi: 10.1002/bmb.20700. Epub 2013 May 8.

DOI:10.1002/bmb.20700

PMID:23653289

Abstract

Terms to be familiar with before you start to solve the test: plasmid, restriction endonuclease, agarose gel electrophoresis, ethidium bromide staining, autoradiography, Coomassie staining, Southern blotting, linear and circular DNA, superhelical DNA, exonuclease, modification methylase, palindrome, sticky and blunt ends, nicked circular DNA.

摘要

在开始解答测试题之前，需要熟悉的术语：质粒、限制性内切酶、琼脂糖凝胶电泳、溴化乙锭染色、放射自显影、考马斯亮蓝染色、Southern印迹法、线性和环状DNA、超螺旋DNA、核酸外切酶、修饰甲基化酶、回文序列、粘性末端和平末端、缺口环状DNA。