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Problem-solving test: Southwestern blotting.

作者信息

Szeberényi József

机构信息

Department of Medical Biology, Medical School, University of Pécs, H-7624, Pécs, Hungary.

出版信息

Biochem Mol Biol Educ. 2014 Sep-Oct;42(5):443-5. doi: 10.1002/bmb.20816. Epub 2014 Aug 5.

Abstract

Terms to be familiar with before you start to solve the test: Southern blotting, Western blotting, restriction endonucleases, agarose gel electrophoresis, nitrocellulose filter, molecular hybridization, polyacrylamide gel electrophoresis, proto-oncogene, c-abl, Src-homology domains, tyrosine protein kinase, nuclear localization signal, cDNA, deletion mutants, expression plasmid, transfection, RNA polymerase II, promoter, Shine-Dalgarno sequence, polyadenylation element, affinity chromatography, Northern blotting, immunoprecipitation, sodium dodecylsulfate, autoradiography, tandem repeats.

摘要

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