离子对高效液相色谱法测定盐酸米托蒽醌注射液

[Ion-paired high performance liquid chromatographic determination of mitoxantrone hydrochloride injection].

作者信息

Wu C, Guo P, Song H

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1990 Mar;21(1):39-41.

Abstract

Mitoxantrone (DHAQ) is a new anti-cancer agent. The ion-pair HPLC method to determine mitoxantrone hydrochloride injection was proposed. Mitoxantrone can be separated on Zorbax-ODS column with methanol-water-formic acid (450:50:1.5) containing 3.605g SDS as mobile phase. The pH of mobile phase was adjusted to 2.7. The flow rate was 1.0 ml/min and detection was at wavelength of 254 nm, providing a detection limit of 1ng (r/n, 3:1) of DHAQ. DHAQ was determined by peak area measurement with ametantrone as internal standard. The rectilinear calibration graph in the range of 1-10 micrograms of DHAQ was obtained (r = 0.999 2). The average recovery was 99.62 +/- 2.36% (n = 10), and the results were not interfered by the other ingredients in the injection.

摘要

米托蒽醌（DHAQ）是一种新型抗癌药。本文提出了离子对高效液相色谱法测定盐酸米托蒽醌注射液的含量。米托蒽醌在Zorbax-ODS柱上，以含3.605g十二烷基硫酸钠的甲醇-水-甲酸（450:50:1.5）为流动相进行分离。流动相pH值调至2.7。流速为1.0ml/min，检测波长为254nm，米托蒽醌的检测限为1ng（信噪比，3:1）。以内标阿霉素通过峰面积测量法测定米托蒽醌。得到了米托蒽醌在1 - 10μg范围内的线性校准曲线（r = 0.9992）。平均回收率为99.62±2.36%（n = 10），注射液中的其他成分不干扰测定结果。