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Repetitive sequence elements in an initiation locus of the amplified dihydrofolate reductase domain in CHO cells.

作者信息

Leu T H, Anachkova B, Hamlin J L

机构信息

Department of Biochemistry and Cell Molecular Biology Program, University of Virginia School of Medicine, Charlottesville 22908.

出版信息

Genomics. 1990 Jul;7(3):428-33. doi: 10.1016/0888-7543(90)90178-w.

DOI:10.1016/0888-7543(90)90178-w
PMID:2365359
Abstract

We have previously demonstrated that one of the replication initiation loci in the dihydrofolate reductase (DHFR) domain of Chinese hamster cells contains a repeated sequence that is enriched in the early-replicating fraction of the Chinese hamster genome. Here we present the sequence of the initiation locus, identify the relevant repeated element, and show that, while this element is enriched in early-replicating DNA, its synthesis is not confined to early S.

摘要

相似文献

1
Repetitive sequence elements in an initiation locus of the amplified dihydrofolate reductase domain in CHO cells.
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2
Replication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites, one of which is a repetitive sequence element.在CHO细胞中，扩增的二氢叶酸还原酶结构域的复制可能在两个不同位点起始，其中一个是重复序列元件。
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The dihydrofolate reductase origin of replication does not contain any nonredundant genetic elements required for origin activity.二氢叶酸还原酶复制起点不包含起点活性所需的任何非冗余遗传元件。
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