University Eye Hospital, Centre for Ophthalmology, Eberhard-Karls University, Tuebingen, Germany.
Curr Eye Res. 2013 Jun;38(6):662-9. doi: 10.3109/02713683.2012.746991.
Abstract Purpose: To evaluate the antiproliferative and cytotoxic properties of moxifloxacin on cultured rat retinal ganglion cells (RGC5).
Rat retinal ganglion cells were exposed to various concentration of moxifloxacin (5-1500 μg/mL). For antiproliferative properties, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) test was performed. Cellular cytotoxicity was assessed by using the Live/Dead viability/cytotoxicity assay and analyzed by fluorescence microscopy after 24 and 72 h of incubation, respectively.
Neither cytotoxic nor antiproliferative effect of moxifloxacin was observed below 50 μg/mL on RGC5 cells after 24 and 72 h of incubation. At higher concentrations of moxifloxacin (150 μg/mL, 500 μg/mL, and 1500 μg/mL (p < 0.001)) the number of viable cells and the proliferation rate of RGC5 were significantly reduced.
These results suggest a dose-dependent cytotoxic and antiproliferative effect of moxifloxacin on RGC5. Therefore, intracameral application of moxifloxacin should be used cautiously in patients with increased risk of retinal ganglion cells damage, particularly in glaucoma patients.
摘要目的：评估莫西沙星对培养的大鼠视网膜神经节细胞（RGC5）的抗增殖和细胞毒性作用。
将大鼠视网膜神经节细胞暴露于不同浓度的莫西沙星（5-1500μg/ml）。为了评估抗增殖特性，进行了 3-（4,5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺苯基）-2H-四唑（MTS）试验。通过 Live/Dead 活力/细胞毒性测定法评估细胞毒性，并在孵育 24 和 72 小时后分别通过荧光显微镜进行分析。
在孵育 24 和 72 小时后，莫西沙星的浓度低于 50μg/ml 时，对 RGC5 细胞既没有细胞毒性也没有抗增殖作用。在较高浓度的莫西沙星（150μg/ml、500μg/ml 和 1500μg/ml（p<0.001））时，RGC5 的存活细胞数量和增殖率显著降低。
这些结果表明莫西沙星对 RGC5 具有剂量依赖性的细胞毒性和抗增殖作用。因此，在有视网膜神经节细胞损伤风险增加的患者中，特别是在青光眼患者中，应谨慎使用玻璃体内应用莫西沙星。
