Trinkaus-Randall V, Capecchi J, Sammon L, Gibbons D, Leibowitz H M, Franzblau C
Boston University School of Medicine, School of Medicine, Ophthalmology Department, MA 02118.
Invest Ophthalmol Vis Sci. 1990 Jul;31(7):1321-6.
We evaluated a polybutylene/polypropylene blend in a blown microfiber configuration in vitro for potential use as the peripheral area of a keratoprosthetic device. Material properties such as ultimate tensile strength and ultimate elongation were measured. Stromal fibroblasts were seeded onto the material in vitro, and cell proliferation between uncoated and Type I collagen coated discs did not differ significantly. Fibroblasts could be seen migrating along the fibers and also traversing the fibers. The synthesis of connective proteins was examined. Laminin, fibronectin, and Type I collagen were detected by day 8. The experiments demonstrate that stromal fibroblasts can adhere onto the fibers, proliferate, and synthesize connective tissue proteins. Experiments are now being conducted to further evaluate the material in vivo.
我们在体外评估了一种吹塑微纤维结构的聚丁烯/聚丙烯共混物，以确定其作为角膜假体装置周边区域的潜在用途。测量了诸如极限拉伸强度和极限伸长率等材料性能。在体外将基质成纤维细胞接种到该材料上，未涂层和I型胶原涂层圆盘之间的细胞增殖没有显著差异。可以看到成纤维细胞沿着纤维迁移并穿过纤维。检测了结缔组织蛋白的合成。在第8天检测到层粘连蛋白、纤连蛋白和I型胶原。这些实验表明，基质成纤维细胞可以附着在纤维上、增殖并合成结缔组织蛋白。目前正在进行实验以进一步在体内评估该材料。
