Trinkaus-Randall V, Banwatt R, Capecchi J, Leibowitz H M, Franzblau C
Boston University School of Medicine, Massachusetts.
Invest Ophthalmol Vis Sci. 1991 Dec;32(13):3245-51.
We have developed a melt-blown fibrous construction of polybutylene/polypropylene in which we previously demonstrated keratocyte ingrowth and collagen synthesis in vitro. In the present studies, we evaluated this material in vivo in interlamellar corneal pockets for periods of up to six months. By day 42, the porous interstices of the disc were heavily populated with keratocytes. Extracellular matrix deposition occurred and there was a 5000-fold increase in total protein and a 1000-fold increase in total collagen over background. The cells within the disc continued to be synthetically active for the six months of our study. Discs remained in corneas for periods of up to one year without any extrusion. This material has great promise as a porous peripheral component of a keratoprosthetic device.
我们研发了一种聚丁烯/聚丙烯熔喷纤维结构，此前我们已在体外证明该结构能促进角膜细胞向内生长并合成胶原蛋白。在本研究中，我们在角膜层间袋内对该材料进行了长达六个月的体内评估。到第42天时，圆盘状材料的多孔间隙中密集分布着角膜细胞。发生了细胞外基质沉积，总蛋白比背景值增加了5000倍，总胶原蛋白增加了1000倍。在我们为期六个月的研究中，圆盘内的细胞一直保持合成活性。圆盘在角膜内留存长达一年，没有任何挤出情况。这种材料作为人工角膜装置的多孔周边部件具有很大的应用前景。
