Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Viral Immunol. 2013 Jun;26(3):201-6. doi: 10.1089/vim.2013.0007. Epub 2013 May 8.
Inflammatory cytokine gene polymorphisms may influence the hepatic and extrahepatic HBV-related disease in transplant patients. In this study, the association between IL-17, IL-23R, and IL-21 gene polymorphisms with hepatitis B virus (HBV) infection was evaluated in kidney transplant patients. In total, 220 kidney transplant patients were enrolled in this cross-sectional study between years 2007 and 2011. The genomic HBV DNA was identified using an HBV PCR detection Kit according to the manufacturer's instruction. The cytokine gene polymorphisms, including IL-17 197 A/G (rs2275913), IL-21 +1472 G/T (rs2055979), IL-21 5250 C/T (rs4833837), and IL-23R C/A (rs10889677) were evaluated by PCR-RFLP and ARMS-PCR protocols. The serum levels of IL-17 and IL-21 were analyzed in HBV infected and noninfected transplant patients by ELISA methods according to manufacturer's instructions. 70 of 220 (35%) transplant patients experienced acute rejection. HBV DNA was detected in 52 of 220 (23.64%) transplant patients. 16 of 52 (30.8%) HBV-infected kidney transplant patients experienced acute rejection. A significant higher frequency of C allele of IL-23R (rs10889677) polymorphism, a higher frequency of AG heterozygote genotype and A allele of IL-17-G197A (rs2275913) polymorphism, a higher frequency of TT homozygote genotype and T allele of IL-21-G1472T (rs2055979) polymorphism, and a higher frequency of CC homozygote genotype and C allele of IL-21-C5250T (rs4833837) polymorphism were found in HBV-infected kidney transplant patients with acute rejection. Diagnosis of the higher frequency of the IL-17, IL-21, and IL-23R cytokine genotypes and allele polymorphisms in HBV-infected kidney transplant patients who experienced acute rejection, illustrates the importance of Th17-related cytokine genetic patterns. A better evaluation of HBV infection in kidney transplant patients is needed.
炎症细胞因子基因多态性可能影响移植患者的肝内和肝外乙型肝炎病毒（HBV）相关疾病。在这项研究中，评估了白细胞介素 17（IL-17）、白细胞介素 23R（IL-23R）和白细胞介素 21（IL-21）基因多态性与乙型肝炎病毒（HBV）感染之间的关系，研究对象为肾移植患者。本横断面研究共纳入 2007 年至 2011 年间的 220 例肾移植患者。根据制造商的说明，使用 HBV PCR 检测试剂盒鉴定基因组 HBV DNA。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和扩增受阻突变系统-PCR（ARMS-PCR）方案评估细胞因子基因多态性，包括白细胞介素 17 197A/G（rs2275913）、白细胞介素 21+1472G/T（rs2055979）、白细胞介素 21 5250C/T（rs4833837）和白细胞介素 23R C/A（rs10889677）。根据制造商的说明，通过酶联免疫吸附试验（ELISA）方法检测 HBV 感染和未感染移植患者的血清白细胞介素 17 和白细胞介素 21 水平。220 例移植患者中有 70 例（35%）发生急性排斥反应。在 220 例移植患者中，有 52 例（23.64%）检测到 HBV DNA。在 52 例 HBV 感染的肾移植患者中，有 16 例（30.8%）发生急性排斥反应。IL-23R（rs10889677）多态性的 C 等位基因、IL-17-G197A（rs2275913）多态性的 AG 杂合基因型和 A 等位基因、IL-21-G1472T（rs2055979）多态性的 TT 纯合基因型和 T 等位基因、IL-21-C5250T（rs4833837）多态性的 CC 纯合基因型和 C 等位基因的频率在 HBV 感染的发生急性排斥反应的肾移植患者中显著更高。在发生急性排斥反应的 HBV 感染肾移植患者中，IL-17、IL-21 和 IL-23R 细胞因子基因型和等位基因多态性的频率较高，这表明 Th17 相关细胞因子遗传模式的重要性。需要对肾移植患者的 HBV 感染进行更好的评估。
