中国人群中白细胞介素-21基因多态性与慢性乙型肝炎感染
Interleukin-21 gene polymorphisms and chronic hepatitis B infection in a Chinese population.
作者信息
Yao Jia-Yan, Chao Kang, Li Min-Rui, Wu Yan-Qing, Zhong Bi-Hui
机构信息
Jia-Yan Yao, Kang Chao, Min-Rui Li, Yan-Qing Wu, Bi-Hui Zhong, Department of Gastroenterology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China.
出版信息
World J Gastroenterol. 2015 Apr 14;21(14):4232-9. doi: 10.3748/wjg.v21.i14.4232.
AIM
To investigate the relationship between interleukin-21 (IL21) gene polymorphisms and chronic hepatitis B virus (HBV) infection in a Chinese population.
METHODS
In this case-control study, 366 Chinese HBV-infected patients were recruited and divided into hepatocellular carcinoma (HCC; n = 94) and non-HCC (n = 272) groups at The First Affiliated Hospital of Sun Yat-Sen University, from April 2009 to December 2012. In the non-HCC group, the patients were classified into three clinical subsets, 76 patients had chronic hepatitis B, 101 were HBV carriers and 95 patients had HBV-related cirrhosis. Two hundred eight unrelated healthy controls were also included. Genomic DNA was extracted from peripheral blood. Single nucleotide polymorphisms (SNPs) rs13143866, rs2221903, and rs907715 were subsequently genotyped using the SNaPshot SNP technique.
RESULTS
There were no significant differences in allele and genotype frequencies of SNPs rs13143866, rs2221903, and rs907715 between chronic HBV-infected patients and control subjects. Furthermore, no significant differences were found in the frequencies of all alleles and genotypes between the HCC group and the non-HCC group. However, in the subgroup analysis, IL21 rs13143866 genotype AA frequency in the HBV carrier group was higher than in controls (OR = 6.280, 95%CI: 1.238-31.854; P = 0.019), and the effect of the recessive model (AA vs GG + GA, OR = 6.505, 95%CI: 1.289-32.828) was observed in the HBV carrier group. IL21 rs2221903 genotype TC frequency in the HBV carrier group was higher than in controls (OR = 1.809, 95%CI: 1.043-3.139; P = 0.035). In the haplotype analysis, the ATA haplotype (rs13143866, rs2221903, and rs907715) of IL21 was more frequent in the HCC group than in the non-HCC group (0.165 vs 0.104, P = 0.044; OR = 1.700, 95%CI: 1.010-2.863).
CONCLUSION
Genotypes rs13143866 AA and rs2221903 TC are risk factors for carrying HBV; ATA haplotype increases the risk of HBV-related HCC onset in a Chinese population.
目的
探讨白细胞介素-21（IL21）基因多态性与中国人群慢性乙型肝炎病毒（HBV）感染之间的关系。
方法
在这项病例对照研究中，2009年4月至2012年12月期间，中山大学附属第一医院招募了366例中国HBV感染患者，并将其分为肝细胞癌（HCC；n = 94）组和非HCC（n = 272）组。在非HCC组中，患者被分为三个临床亚组，76例为慢性乙型肝炎患者，101例为HBV携带者，95例为HBV相关肝硬化患者。还纳入了208名无血缘关系的健康对照。从外周血中提取基因组DNA。随后使用SNaPshot SNP技术对单核苷酸多态性（SNP）rs13143866、rs2221903和rs907715进行基因分型。
结果
慢性HBV感染患者与对照受试者之间，SNP rs13143866、rs2221903和rs907715的等位基因和基因型频率无显著差异。此外，HCC组与非HCC组之间所有等位基因和基因型的频率也未发现显著差异。然而，在亚组分析中，HBV携带者组中IL21 rs13143866基因型AA频率高于对照组（OR = 6.280，95%CI：1.238 - 31.854；P = 0.019），并且在HBV携带者组中观察到隐性模型（AA与GG + GA相比，OR = 6.505，95%CI：1.289 - 32.828）的效应。HBV携带者组中IL21 rs2221903基因型TC频率高于对照组（OR = 1.809，95%CI：1.043 - 3.139；P = 0.035）。在单倍型分析中，IL21的ATA单倍型（rs13143866、rs2221903和rs907715）在HCC组中比非HCC组更常见（0.165对0.104，P = 0.044；OR = 1.700，95%CI：1.010 - 2.863）。
结论
基因型rs13143866 AA和rs2221903 TC是携带HBV的危险因素；ATA单倍型增加了中国人群中HBV相关HCC发病的风险。
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