Zhang Chunting, Su Ping, Wang Siming, Ding Fangyu, Yang Yang, Yang Yi
College of Science, Beijing University of Chemical Technology, Beijing, PR China.
J Immunoassay Immunochem. 2013;34(3):246-54. doi: 10.1080/15321819.2012.704473.
Polyamidoamine dendrimer (PAMAM) is one of a number of dendritic polymers with precise molecular structure, highly geometric symmetry, and a large number of terminal groups. The polyamidoamine modified silica was synthesized with microwave assisted protocol. Anti-epitestosterone monoclonal antibodies were immobilized onto the PAMAM grafted silica and prepared an off-line immunoextraction column that applied in the extraction of testosterone and epitestosterone. The results showed that the affinity activity of the anti-epitestosterone monoclonal antibodies was remained at high level after immobilization. It was satisfactory to apply this new type of immunoextraction column to analyze testosterone and epitestosterone in spiked urine sample.
聚酰胺-胺树枝状大分子（PAMAM）是众多具有精确分子结构、高度几何对称性和大量端基的树枝状聚合物之一。采用微波辅助方法合成了聚酰胺-胺修饰的二氧化硅。将抗表睾酮单克隆抗体固定在接枝有PAMAM的二氧化硅上，制备了一种离线免疫萃取柱，用于睾酮和表睾酮的萃取。结果表明，抗表睾酮单克隆抗体固定后仍保持较高的亲和活性。将这种新型免疫萃取柱应用于加标尿液样本中睾酮和表睾酮的分析，结果令人满意。
