He Chiyang, Li Shehong, Liu Huwei, Li Kean, Liu Feng
The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry, Peking University, Chengfu Road, Beijing 100871, China.
J Chromatogr A. 2005 Aug 5;1082(2):143-9. doi: 10.1016/j.chroma.2005.05.065.
Based on aqueous two-phase systems (ATPS) consisting of 1-butyl-3-methylimidazolium chloride, a hydrophilic ionic liquid (IL), and K2HPO4, a new and simple extraction technique, coupled with a reversed-phase high performance liquid chromatography (RP-HPLC), was developed for the simultaneous concentration and analysis of testosterone (T) and epitestosterone (ET) in human urine. Under the optimal conditions, the extraction efficiencies for both analytes were 80-90% in a one-step extraction. The method required only 3.0 mL of urine and a single hydrolysis/deproteinization/extraction step followed by direct injection of the IL-rich upper phase into HPLC system for analysis. The method has been satisfactorily applied to the analysis of T and ET in human urine with detection limits of 1 ng/mL and linear ranges of 10-500 ng/mL for both compounds. Compared with conventional liquid-liquid extraction or solid phase extraction, this new method is much "greener" due to no use of volatile organic solvent and low consumption of IL. The proposed extraction technique opens up new possibilities in the separation of other drugs.
基于由亲水性离子液体1-丁基-3-甲基咪唑氯盐和磷酸氢二钾组成的双水相体系（ATPS），开发了一种新的简单萃取技术，并与反相高效液相色谱（RP-HPLC）联用，用于同时浓缩和分析人尿中的睾酮（T）和表睾酮（ET）。在最佳条件下，一步萃取时两种分析物的萃取效率均为80-90%。该方法仅需3.0 mL尿液，经过单一的水解/脱蛋白/萃取步骤，然后将富含离子液体的上相直接注入HPLC系统进行分析。该方法已成功应用于人尿中T和ET的分析，两种化合物的检测限均为1 ng/mL，线性范围均为10-500 ng/mL。与传统的液-液萃取或固相萃取相比，该新方法由于不使用挥发性有机溶剂且离子液体消耗低，因而更加“绿色”。所提出的萃取技术为其他药物的分离开辟了新的可能性。
