Henderson K A, Cupps P T
University of California, Davis 95616.
J Anim Sci. 1990 May;68(5):1363-9. doi: 10.2527/1990.6851363x.
Acid and alkaline phosphatases were measured in the follicular fluid of 766 individual follicles from 96 cows. Follicles were obtained by bilateral ovariectomy or at slaughter from animals at various stages of the estrous cycle and pregnancy. Mean follicle size varied with the physiological state of the cow (P less than .0001). Acid phosphatase activity (U/microliters) varied inversely with follicle size (P less than .001) but not with stage of the estrous cycle or gestation. Total acid phosphatase activity per follicle increased with follicle size (P less than .05). Neither acid phosphatase nor alkaline phosphatase concentration was associated with atresia. Alkaline phosphatase activity (U/microliters) was greater in the smallest follicles (less than 50 microliters) than in other size groups (P less than .0001). Alkaline phosphatase activity (U/microliters) was greater (P less than .05) during the preovulatory phase of the estrous cycle than during other phases. A high concentration of follicular fluid phosphatases cannot be used as a marker for atresia but is characteristic of healthy small antral follicles.
对来自96头奶牛的766个卵泡的卵泡液中的酸性磷酸酶和碱性磷酸酶进行了测定。卵泡通过双侧卵巢切除术或在动物发情周期和妊娠的不同阶段屠宰时获取。平均卵泡大小随奶牛的生理状态而变化（P<0.0001）。酸性磷酸酶活性（U/微升）与卵泡大小呈负相关（P<0.001），但与发情周期或妊娠阶段无关。每个卵泡的总酸性磷酸酶活性随卵泡大小增加（P<0.05）。酸性磷酸酶和碱性磷酸酶浓度均与闭锁无关。最小的卵泡（小于50微升）中的碱性磷酸酶活性（U/微升）高于其他大小组（P<0.0001）。发情周期的排卵前期的碱性磷酸酶活性（U/微升）高于其他阶段（P<0.05）。高浓度的卵泡液磷酸酶不能用作闭锁的标志物，但它是健康小窦卵泡的特征。
