Department of Pneumonology, Aristotle University of Thessaloniki, Georgios Papanikolaou Hospital, Thessaloniki, Greece.
J Interferon Cytokine Res. 2013 May;33(5):261-9. doi: 10.1089/jir.2012.0022.
In pulmonary sarcoidosis, differential cytokine production in the lungs could be related to variable prognosis of patients at different stages of disease. Twenty patients with pulmonary sarcoidosis (10 at radiographic stage I and 10 at stages II-IV), as well as 10 age-matched healthy volunteers participated in the study. A 4-colour flow cytometric technique was used to measure interferon-γ (IFN-γ), interleukin (IL)-2, tumour necrosis factor-α (TNF-α), IL-4, and IL-13 production in phorbol myristate acetate (PMA)/ionomycin-stimulated CD4+ and CD8+ T cells from bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) of patients, and PB of control subjects. CD4+ T cells from patients showed higher expression of IFN-γ in BALF than in PB. Significant correlations were observed between the percentages of BALF CD4+ and CD8+ T cells expressing intracellular IFN-γ, IL-2, and TNF-α. Stage I patients had lower percentages of IFN-γ-producing CD4+ and CD8+ T cells, as well as TNF-α-producing CD8+ T cells, in BALF (but not in PB) than stage II-IV patients. A decreased TH1 and TC1 response was demonstrated in BALF of patients at stage I of disease, which could explain their anticipated better prognosis.
在肺结节病中，肺部差异细胞因子的产生可能与疾病不同阶段患者的不同预后有关。本研究纳入了 20 名肺结节病患者（10 名处于放射学 I 期，10 名处于 II-IV 期）和 10 名年龄匹配的健康志愿者。采用四色流式细胞术检测博来霉素（PMA）/离子霉素刺激的支气管肺泡灌洗液（BALF）和外周血（PB）中 CD4+和 CD8+T 细胞以及对照组 PB 中 IFN-γ、IL-2、肿瘤坏死因子-α（TNF-α）、IL-4 和 IL-13 的产生情况。与 PB 相比，患者的 BALF 中 CD4+T 细胞 IFN-γ表达更高。BALF 中 CD4+和 CD8+T 细胞内 IFN-γ、IL-2 和 TNF-α的表达百分比之间存在显著相关性。与 II-IV 期患者相比，I 期患者的 BALF 中 IFN-γ产生的 CD4+和 CD8+T 细胞以及 TNF-α产生的 CD8+T 细胞比例较低。疾病 I 期患者 BALF 中的 TH1 和 TC1 反应减弱，这可以解释他们预期的更好预后。
