Institut National de la Recherche Agronomique , UR1067 Nutrition Métabolisme Aquaculture, St-Pée-sur-Nivelle, France.
Zebrafish. 2013 Jun;10(2):237-48. doi: 10.1089/zeb.2012.0835. Epub 2013 May 9.
Zebrafish (Danio rerio) have been proposed as a possible model organism for nutritional physiology. However, this potential has not yet been realized and studies on the field remain scarce. In this work, we investigated in this species the effect of a single meal as well as that of an increase in the ratio of dietary carbohydrates/proteins on the postprandial expression of several hepatic and muscle metabolism-related genes and proteins. Fish were fed once either a commercial diet (experiment 1) or one of two experimental diets (experiment 2) containing different protein and carbohydrate levels after 72 h of starvation. Refeeding induced the postprandial expression of genes of glycolysis (GK, HK1) and lipogenesis (FAS, G6PDH, ACCa) and inhibited those of gluconeogenesis (cPEPCK) and beta-oxidation (CPT1b) in the viscera. In the muscle, refeeding increased transcript levels of myogenesis (Myf5, Myogenin), inhibited those of Ub-proteasomal proteolytic system (Atrogin1, Murf1a, Murf1b), and induced the activation of key signaling factors of protein synthesis (Akt, 4EBP1, S6K1, S6). However, diet composition had a low impact on the studied factors. Together, these results highlight some specificity of the zebrafish metabolism and demonstrate the interest and the limits of this species as a model organism for nutritional physiology studies.
斑马鱼（Danio rerio）被提议作为营养生理学的潜在模式生物。然而，这种潜力尚未实现，该领域的研究仍然很少。在这项工作中，我们研究了该物种单次摄食以及增加饮食中碳水化合物/蛋白质比例对餐后肝脏和肌肉代谢相关基因和蛋白质表达的影响。在饥饿 72 小时后，鱼要么单次喂食商业饮食（实验 1），要么喂食两种含有不同蛋白质和碳水化合物水平的实验饮食（实验 2）。再喂食诱导了内脏中糖酵解（GK、HK1）和脂肪生成（FAS、G6PDH、ACCa）基因的餐后表达，并抑制了糖异生（cPEPCK）和β-氧化（CPT1b）基因的表达。在肌肉中，再喂食增加了肌肉生成（Myf5、Myogenin）的转录水平，抑制了泛素-蛋白酶体蛋白水解系统（Atrogin1、Murf1a、Murf1b）的基因表达，并诱导了蛋白质合成关键信号因子的激活（Akt、4EBP1、S6K1、S6）。然而，饮食组成对研究因素的影响很小。总的来说，这些结果强调了斑马鱼代谢的一些特异性，并证明了该物种作为营养生理学研究模型的兴趣和局限性。
