Marandel Lucie, Plagnes-Juan Elisabeth, Marchand Michael, Callet Therese, Dias Karine, Terrier Frederic, Père Stéphane, Vernier Louise, Panserat Stephane, Rétaux Sylvie
INRAE, Université de Pau & Pays de l'Adour, E2S UPPA, UMR1419 Nutrition Metabolism and Aquaculture, Aquapôle, 64310 Saint-Pée-sur-Nivelle, France.
Paris-Saclay Institute of Neuroscience, CNRS UMR9197, Université Paris-Saclay, Avenue de la terrasse, Gif-sur-Yvette, France.
R Soc Open Sci. 2020 Feb 26;7(2):191853. doi: 10.1098/rsos.191853. eCollection 2020 Feb.
has gained importance as a laboratory model organism for evolutionary biology. However, little is known about its intermediary metabolism, and feeding regimes remain variable between laboratories holding this species. We thus aimed to evaluate the intermediary metabolism response to nutritional status and to low (NC) or high (HC) carbohydrate diets in various organs of the surface-dwelling form of the species. As expected, glycaemia increased after feeding. Fish fed the HC diet had higher glycaemia than fish fed the NC diet, but without displaying hyperglycaemia, suggesting that carbohydrates are efficiently used as an energy source. At molecular level, only () transcripts increased in tissues after refeeding, suggesting an activation of lipogenesis. On the other hand, we monitored only moderate changes in glucose-related transcripts. Most changes observed were related to the nutritional status, but not to the NC versus HC diet. Such a metabolic pattern is suggestive of an omnivorous-related metabolism, and this species, at least at adult stage, may adapt to a fish meal-substituted diet with high carbohydrate content and low protein supply. Investigation to identify molecular actors explaining the efficient use of such a diet should be pursued to deepen our knowledge on this species.
作为进化生物学的实验室模式生物，它已变得越发重要。然而，人们对其中间代谢了解甚少，而且在饲养该物种的不同实验室之间，喂养方式仍然存在差异。因此，我们旨在评估该物种地表生活形态的各种器官对营养状况以及低（NC）或高（HC）碳水化合物饮食的中间代谢反应。正如预期的那样，喂食后血糖升高。喂食HC饮食的鱼比喂食NC饮食的鱼血糖更高，但未出现高血糖，这表明碳水化合物能有效地用作能量来源。在分子水平上，再喂食后组织中只有（）转录本增加，表明脂肪生成被激活。另一方面，我们仅监测到与葡萄糖相关的转录本有适度变化。观察到的大多数变化与营养状况有关，而非与NC饮食和HC饮食有关。这种代谢模式表明其具有杂食性相关的代谢，并且该物种至少在成年阶段可能适应高碳水化合物含量和低蛋白质供应的鱼粉替代饮食。应继续进行研究以确定解释这种饮食有效利用的分子因素，从而加深我们对该物种的了解。
