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甘蓝型油菜-白菜型油菜单体异附加系的创制与鉴定。

Generation and identification of Brassica alboglabra-Brassica campestris monosomic alien addition lines.

机构信息

College of Horticulture, Agricultural University of Hebei, Baoding 071001, China.

出版信息

Genome. 2013 Mar;56(3):171-7. doi: 10.1139/gen-2012-0139. Epub 2013 Mar 13.

DOI:10.1139/gen-2012-0139
PMID:23659701
Abstract

Four monosomic alien addition lines (MAALs) for Brassica alboglabra-Brassica campestris were developed through digenomic triploid (ACC) backcrossing with the recurrent parent B. alboglabra (CC). The objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. Based on the new chromosome nomenclature system established for Brassica, we preliminarily identified these MAALs as CC+A1, CC+A3, CC+A6, and CC+A7. Their alien chromosomes were transmittable through both female and male gametes at rates of 11.46%-26.53% and 4.88%-12.90%, respectively.

摘要

四个二倍体-四倍体衍生的芥菜型油菜-白菜型油菜单体附加系(MAALs)通过二倍体种(ACC)回交与轮回亲本芥菜型油菜(CC)创制。本研究的目的是比较形态特征、微卫星标记(简单序列重复)、染色体核型和减数分裂行为。基于建立的芸薹属新染色体命名系统,我们初步鉴定这些 MAALs 为 CC+A1、CC+A3、CC+A6 和 CC+A7。它们的异源染色体可以通过雌配子和雄配子传递,传递率分别为 11.46%-26.53%和 4.88%-12.90%。

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