Gu Ai Xia, Shen Shu Xing, Wang Yan Hua, Zhao Jian Jun, Xuan Shu Xin, Chen Xue Ping, Li Xiao Feng, Luo Shuang Xia, Zhao Yu Jing
College of Horticulture, Agricultural University of Hebei, No. 289, Lingyusi Road, Baoding 071001, People's Republic of China.
J Genet. 2015 Sep;94(3):435-44. doi: 10.1007/s12041-015-0542-9.
Five monosomic alien addition lines (MAALs) of Brassica rapa ssp. pekinensis - B. oleracea var. capitata were obtained by hybridization and backcrossing between B. rapa ssp. pekinensis (female parent) and B. oleracea var. capitata. The alien linkage groups were identified using 42 B. oleracea var. capitata linkage group-specific markers as B. oleracea linkage groups C2, C3, C6, C7 and C8. Based on the chromosomal karyotype of root tip cells, these five MAALs added individual chromosomes from B. oleracea var. capitata: chr 1 (the longest), chr 2 or 3, chr 5 (small locus of 25S rDNA), chr 7 (satellite-carrying) and chr 9 (the shortest). Five disomic alien addition lines were then generated by selfing their corresponding MAALs.
通过白菜型油菜（母本）与甘蓝的杂交和回交，获得了5个白菜型油菜-甘蓝单体异附加系。利用42个甘蓝连锁群特异性标记，将外源连锁群鉴定为甘蓝的连锁群C2、C3、C6、C7和C8。根据根尖细胞的染色体核型，这5个单体异附加系分别添加了来自甘蓝的单条染色体：1号染色体（最长）、2号或3号染色体、5号染色体（25S rDNA小位点）、7号染色体（携带卫星）和9号染色体（最短）。然后通过其相应的单体异附加系自交，产生了5个二体异附加系。
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