Department of Medical Biology, Jagiellonian University College of Medicine, Kraków, Poland.
Immunology. 2013 Sep;140(1):98-110. doi: 10.1111/imm.12120.
Natural killer (NK) cell-mediated contact sensitivity was recently described in mice. Here, we confirm NK cell-mediated contact sensitivity (CS) in SCID and RAG1(-/-) mice but not in SCIDbeige mice, which have non-functional NK cells that lack NK cell granules. NK cell-mediated CS was transferred by liver mononuclear cells and the DX5(+) fraction of liver cells, confirming that NK cells mediate CS in the absence of T and B cells. Participation of NKT cells and B-1 cells was ruled out using Jα18(-/-) and JH(-/-) mice, respectively. Remarkably, NK cell-mediated CS was observed just 1 hr after immunization and was detectable as early as 30 min after challenge. Further, we examined cytokine requirements for NK cell-mediated CS, and found that liver mononuclear cells from interleukin-12(-/-) , interferon-γ(-/-) and interferon-α receptor(-/-) donors fail to transfer NK cell-mediated CS to naive hosts. Our studies clearly show that dinitrofluorobenzene sensitized NK cells mediate very rapid, antigen-specific cell-mediated immunity, with features of both innate and acquired immune responses.
自然杀伤 (NK) 细胞介导的接触敏感性最近在小鼠中被描述。在这里，我们在 SCID 和 RAG1(-/-) 小鼠中证实了 NK 细胞介导的接触敏感性 (CS)，但在缺乏 NK 细胞颗粒且 NK 细胞功能失调的 SCIDbeige 小鼠中未观察到。NK 细胞介导的 CS 可通过肝单核细胞和肝细胞的 DX5(+) 部分转移，证实 NK 细胞在没有 T 和 B 细胞的情况下介导 CS。使用 Jα18(-/-) 和 JH(-/-) 小鼠分别排除了 NKT 细胞和 B-1 细胞的参与。值得注意的是，NK 细胞介导的 CS 在免疫后仅 1 小时即可观察到，在挑战后 30 分钟即可检测到。此外，我们检查了 NK 细胞介导的 CS 的细胞因子要求，发现来自白细胞介素-12(-/-)、干扰素-γ(-/-)和干扰素-α受体(-/-)供体的肝单核细胞不能将 NK 细胞介导的 CS 转移到未致敏的宿主。我们的研究清楚地表明，二硝基氟苯致敏的 NK 细胞介导非常快速的、抗原特异性的细胞介导免疫，具有先天和获得性免疫反应的特征。
