Iowa State University, Department of Animal Science, Ames, IA, United States of America.
USDA-ARS-National Animal Disease Center, Infectious Bacterial Diseases of Livestock Research Unit, Ames, IA, United States of America.
PLoS One. 2019 Oct 9;14(10):e0223483. doi: 10.1371/journal.pone.0223483. eCollection 2019.
Hapten contact hypersensitivity (CHS) elicits a well-documented inflammation response that can be used to illustrate training of immune cells through hapten-specific CHS memory. The education of hapten-specific memory T cells has been well-established, recent research in mice has expanded the "adaptive" characteristic of a memory response from solely a function of the adaptive immune system, to innate cells as well. To test whether similar responses are seen in a non-rodent model, we used hapten-specific CHS to measure the ear inflammation response of outbred pigs to dinitrofluorobenzene (DNFB), oxazolone (OXA), or vehicle controls. We adapted mouse innate memory literature protocols to the domestic pig model. Animals were challenged up to 32 days post initial sensitization exposure to the hapten, and specific ear swelling responses to this challenge were significant for 7, 21, and 32 days post-sensitization. We established hapten-specific CHS memory exists in a non-rodent model. We also developed a successful protocol for demonstrating these CHS responses in a porcine system.
半抗原接触超敏反应 (CHS) 会引起有据可查的炎症反应，可用于说明通过半抗原特异性 CHS 记忆对免疫细胞进行训练。半抗原特异性记忆 T 细胞的教育已经得到很好的建立，最近在小鼠中的研究将记忆反应的“适应性”特征从仅适用于适应性免疫系统扩展到固有细胞。为了测试在非啮齿动物模型中是否存在类似的反应，我们使用半抗原特异性 CHS 来测量杂种猪对二硝基氟苯 (DNFB)、恶唑酮 (OXA) 或载体对照的耳炎症反应。我们将小鼠固有记忆文献方案改编为家猪模型。在最初致敏暴露于半抗原后，动物最多可接受 32 天的挑战，并且在致敏后 7、21 和 32 天，对此类挑战的特定耳部肿胀反应具有显著意义。我们确定半抗原特异性 CHS 记忆存在于非啮齿动物模型中。我们还开发了一种成功的方案，用于在猪系统中证明这些 CHS 反应。
