Liu Xin-Xing, Zhang Yu-Tian, Zhang Bo
College of Life Sciences, Peking University, Beijing, China.
Yi Chuan. 2013 Apr;35(4):529-32. doi: 10.3724/sp.j.1005.2013.00529.
In recent years, zebrafish has been found to be strikingly capable of heart regeneration at adult stage, which sheds lights on cardiac regenerative medicine, and has also become an important direction for the study of vertebrate genetic and developmental mechanisms. Dissecting the regeneration process and unraveling the underlying molecular and cellular mechanisms might help recover the regeneration capacity of the mammalian heart and further provide instructions to potential therapies for certain cardiac diseases such as myocardial infarction. Here, we introduced a simple surgical method to construct heart injury and regeneration model in zebrafish through amputation of ~20% ventricle. The main procedure includes anaesthetization of adult zebrafish, exposure of the heart by dissecting the nearby abdominal skin and opening the pericardium, and amputation of certain fraction of the ventricle at the cardiac apex under a stereo microscope. Over 90% success rate and easy handling with high reproducibility enable this method to be the most commonly used one for the study of zebrafish heart regeneration.
近年来，人们发现斑马鱼在成年阶段具有惊人的心脏再生能力，这为心脏再生医学带来了曙光，也成为研究脊椎动物遗传和发育机制的重要方向。剖析再生过程并揭示其潜在的分子和细胞机制，可能有助于恢复哺乳动物心脏的再生能力，并进一步为某些心脏病（如心肌梗死）的潜在治疗提供指导。在此，我们介绍一种简单的手术方法，通过切除约20%的心室来构建斑马鱼心脏损伤和再生模型。主要步骤包括对成年斑马鱼进行麻醉，通过解剖附近的腹部皮肤并打开心包来暴露心脏，以及在立体显微镜下于心脏顶端切除一定比例的心室。超过90%的成功率以及易于操作且具有高重复性，使得该方法成为研究斑马鱼心脏再生最常用的方法。
