Sheng Delicia Z, Zheng Dawei, Kikuchi Kazu
Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia.
St. Vincent's Clinical School, University of New South Wales, Kensington, NSW, Australia.
Methods Mol Biol. 2021;2158:63-69. doi: 10.1007/978-1-0716-0668-1_6.
The zebrafish (Danio rerio) possesses a spectacular capacity for cardiac regeneration. Zebrafish have been used in cardiac regeneration research for nearly two decades, contributing to the identification of signals and cellular mechanisms as potential targets for human heart repair. Investigations into cardiac regeneration in zebrafish have been facilitated by multiple methods of inducing cardiac tissue damage. Among the established methods, cardiac resection injury is a relatively simple, yet robust approach traditionally used to induce cardiac tissue damage in a reproducible manner. Here, we describe a detailed protocol to perform a cardiac resection injury in adult zebrafish and discuss potential complications for researchers who are new to this technique.
斑马鱼（Danio rerio）具有惊人的心脏再生能力。近二十年来，斑马鱼一直被用于心脏再生研究，有助于识别作为人类心脏修复潜在靶点的信号和细胞机制。多种诱导心脏组织损伤的方法推动了对斑马鱼心脏再生的研究。在已确立的方法中，心脏切除损伤是一种相对简单但可靠的方法，传统上用于以可重复的方式诱导心脏组织损伤。在此，我们描述了在成年斑马鱼中进行心脏切除损伤的详细方案，并讨论了初次使用该技术的研究人员可能遇到的并发症。
