Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Ecotoxicol Environ Saf. 2013 Jul;93:128-34. doi: 10.1016/j.ecoenv.2013.03.035. Epub 2013 May 6.
The potential of Hydrocotyle vulgaris as an aquatic plant species was evaluated for phytoremediation of C.I. Basic Red 46 (BR46) from nutrient solution. Under the optimized experimental conditions, BR46 was removed up to 95% from incubation medium by H. vulgaris. The ability of the plant in consecutive removal under long term repetitive experiments confirmed the biodegradation process. Accordingly, a number of produced intermediate compounds were identified. An artificial neural network (ANN) model was developed to predict the biodegradation efficiency. A predictive performance (R(2)=0.974) was obtained based on the network results. Interestingly, dye stress enhanced the activity of antioxidant enzymes including superoxide dismutase, peroxidase and catalase in H. vulgaris roots and leaves. Enzymatic responses found to be highly depended on the plant organ and dye concentration in the liquid medium. Overall, the increase in the activity of antioxidant enzymes was much higher in the roots than in the leaves. Nevertheless, no significant increase in the malondialdehyde (MDA) content was detected in both roots and leaves which reflects the high efficiency of antioxidant system in the elimination of reactive oxygen species.
水车前作为一种水生植物，其对 C.I. 碱性红 46（BR46）的修复潜力在从营养溶液中去除 BR46 方面得到了评估。在优化的实验条件下，BR46 被水车前从孵育介质中去除了高达 95%。植物在长期重复实验中的连续去除能力证实了生物降解过程。因此，鉴定出了一些产生的中间化合物。开发了人工神经网络（ANN）模型来预测生物降解效率。基于网络结果，获得了良好的预测性能（R(2)=0.974）。有趣的是，染料胁迫增强了抗氧化酶的活性，包括超氧化物歧化酶、过氧化物酶和过氧化氢酶，在水车前的根和叶中。酶促反应发现高度依赖于植物器官和液体培养基中的染料浓度。总的来说，抗氧化酶的活性在根中比在叶中增加得更高。然而，在根和叶中均未检测到丙二醛（MDA）含量的显著增加，这反映了抗氧化系统在消除活性氧方面的高效率。
