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利用钝顶螺旋藻同时提高 C-藻蓝蛋白产量和 CO2 固定的工程策略。

Engineering strategies for simultaneous enhancement of C-phycocyanin production and CO2 fixation with Spirulina platensis.

机构信息

University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.

出版信息

Bioresour Technol. 2013 Oct;145:307-12. doi: 10.1016/j.biortech.2013.01.054. Epub 2013 Jan 18.

DOI:10.1016/j.biortech.2013.01.054
PMID:23664178
Abstract

Spirulina platensis produces nutraceutical product C-phycocyanin (C-PC) and simultaneously mitigates CO2 emissions during its growth. Using a designed flat-type photobioreactor, the S. platensis biomass production was markedly enhanced, leading to a CO2 removal rate and a biomass concentration of 0.23 g/L/d and 2.25 g/L, respectively. The cell growth, CO2 fixation rate and C-PC production of S. platensis were investigated when it was cultivated under different irradiation conditions. As the light intensity increased from 100 to 700 μmol/m(2)/s, the overall biomass productivity, CO2 consumption rate and maximal C-PC productivity increased significantly to 0.74, 1.53 and 0.11 g/L/d, respectively. After determining the suitable light intensity, the nitrogen concentration was also adjusted to further enhance the performance of CO2 fixation and C-PC production. The results show that with an optimal nitrogen concentration of 0.045 M, the CO2 consumption rate and maximal C-PC productivity were further increased to 1.58 and 0.13 g/L/d, respectively.

摘要

螺旋藻生产的营养保健品藻蓝蛋白(C-PC),同时减轻其生长过程中的二氧化碳排放量。使用设计的平板式光生物反应器,明显提高了螺旋藻生物质产量,导致二氧化碳去除率和生物质浓度分别达到 0.23 g/L/d 和 2.25 g/L。研究了不同辐照条件下培养螺旋藻时的细胞生长、二氧化碳固定率和 C-PC 生产情况。随着光强从 100 增加到 700 μmol/m(2)/s,总生物质生产力、二氧化碳消耗率和最大 C-PC 生产力分别显著提高到 0.74、1.53 和 0.11 g/L/d。确定合适的光强后,还调整了氮浓度以进一步提高二氧化碳固定和 C-PC 生产的性能。结果表明,在最佳氮浓度为 0.045 M 时,二氧化碳消耗率和最大 C-PC 生产力进一步提高到 1.58 和 0.13 g/L/d。

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