Zhu Baohua, Shen Han, Li Yun, Liu Qiuke, Jin Guiyong, Han Jichang, Zhao Yan, Pan Kehou
Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao, China.
College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Front Bioeng Biotechnol. 2020 Jan 9;7:441. doi: 10.3389/fbioe.2019.00441. eCollection 2019.
In order to select excellent strains with high CO fixation capability on a large scale, nine species were cultivated in columnar photobioreactors with the addition of 10% CO. The two species selected (208 and 220) were optimized for pH value, total dissolved inorganic carbon (DIC), and phosphorus content with intermittent CO addition in 4 m indoor raceway ponds. On the basis of biomass accumulation and CO fixation rate in the present study, the optimum pH, DIC, and phosphate concentration were 9.5, 0.1 mol L, and 200 mg L for both strains, respectively. Lastly, the two strains selected were semi-continuously cultivated successfully for CO mitigation in 605 m raceway ponds aerated with food-grade CO purified from a coal chemical flue gas on a large scale. The daily average biomass dry weight of the two stains reached up to 18.7 and 13.2 g m d, respectively, suggesting the two strains can be utilized for mass production.
为了大规模筛选出具有高CO固定能力的优良菌株，在添加10% CO的柱状光生物反应器中培养了9个物种。所选的两个物种（208和220）在4米的室内跑道池中通过间歇性添加CO对pH值、总溶解无机碳（DIC）和磷含量进行了优化。基于本研究中的生物量积累和CO固定率，两种菌株的最佳pH值、DIC和磷酸盐浓度分别为9.5、0.1 mol/L和200 mg/L。最后，所选的两种菌株在605米的跑道池中成功进行了半连续培养，该跑道池用从煤化工厂烟道气中提纯的食品级CO进行曝气，以实现大规模的CO减排。两种菌株的日均生物量干重分别达到18.7和13.2 g/m²·d，表明这两种菌株可用于大规模生产。
