Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, Bloco 16, 05508-900 São Paulo-SP, Brazil.
Biotechnol Bioeng. 2012 Feb;109(2):444-50. doi: 10.1002/bit.23315. Epub 2011 Sep 9.
Arthrospira platensis was cultivated in tubular photobioreactor in order to evaluate growth and biomass production at variable photosynthetic photon flux density (PPFD = 60, 120, and 240 µmol photons m(-2)s(-1)) and employing three different systems for cell circulation, specifically an airlift, a motor-driven pumping and a pressurized system. The influence of these two independents variables on the maximum cell concentration (X(m)), cell productivity (P(x)), nitrogen-to-cell conversion factor (Y(X/N) ), photosynthetic efficiency (PE), and biomass composition (total lipids and proteins), taken as responses, was evaluated by analysis of variance. The statistical analysis revealed that the best combination of responses' mean values (X(m) = 4,055 mg L(-1), P(x) = 406 mg L(-1)day(-1), Y(X/N) = 5.07 mg mg(-1), total lipids = 8.94%, total proteins = 30.3%, PE = 2.04%) was obtained at PPFD = 120 µmol photons m(-2)s(-1); therefore, this light intensity should be considered as the most well-suited for A. platensis cultivation in this photobioreactor configuration. The airlift system did not exert any significant positive statistical influence on the responses, which suggests that this traditional cell circulation system could successfully be substituted by the others tested in this work.
为了评估在不同光合光子通量密度（PPFD=60、120 和 240 μmol 光子 m(-2)s(-1)）下的生长和生物量生产，在管状光生物反应器中培养了螺旋藻。采用三种不同的细胞循环系统，即气升式、电动泵送和加压系统，对细胞循环进行了研究。通过方差分析评估了这两个独立变量对最大细胞浓度（X(m)）、细胞生产力（P(x)）、氮到细胞的转化因子（Y(X/N)）、光合作用效率（PE）和生物量组成（总脂质和蛋白质）的影响。统计分析表明，在 PPFD=120 μmol 光子 m(-2)s(-1)下，获得了响应的最佳平均值组合（X(m)=4055 mg L(-1)，P(x)=406 mg L(-1)day(-1)，Y(X/N)=5.07 mg mg(-1)，总脂质=8.94%，总蛋白质=30.3%，PE=2.04%）；因此，应将该光强度视为该光生物反应器构型中最适合 A. platensis 培养的光强度。气升式系统对响应没有施加任何显著的正统计影响，这表明这种传统的细胞循环系统可以成功地被本文测试的其他系统所替代。
