配体诱导的 RNA 假结形成。

Ligand-inducible formation of RNA pseudoknot.

机构信息

Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.

出版信息

Bioorg Med Chem Lett. 2013 Jun 15;23(12):3539-41. doi: 10.1016/j.bmcl.2013.04.037. Epub 2013 Apr 22.

DOI:10.1016/j.bmcl.2013.04.037

PMID:23664873

Abstract

Here, we demonstrate that a series of naphthyridine derivatives, naphthyridine carbamate tetramer (NCTn), can bind to the RNA CGG/CGG triad comprised of two single-stranded regions of a hairpin loop and a tail. Complete suppression of the binding by a single mutation indicated simultaneous binding of NCTn between hairpin loop and single stranded tail, leading to the formation of NCTn-induced pseudoknot.

摘要

在这里，我们证明了一系列的萘啶衍生物，萘啶基氨基甲酸四聚体（NCTn），可以与 RNA CGG/CGG 三链体结合，该三链体由发夹环的两个单链区和一个尾巴组成。单个突变的完全抑制表明 NCTn 可以同时结合在发夹环和单链尾巴之间，导致 NCTn 诱导的假结的形成。