Verschuren P M, Houtsmuller U M, Zevenbergen J L
Unilever Research Laboratorium Vlaardingen, The Netherlands.
Lab Anim. 1990 Apr;24(2):164-71. doi: 10.1258/002367790780890167.
The vitamin E requirement of rabbits fed a semi-synthetic diet containing high amounts of fish oil was studied. Three groups of 5 rabbits were fed fish oil diets containing, respectively, 50, 100 and 500 mg/kg vitamin E. Moreover diet palatability was evaluated by using different levels of grass meal: 0.5, 1 and 2%, respectively. Incorporation of 1% grass meal in the diet was sufficient to achieve acceptance of the fish oil diet. Increased vitamin E intake resulted in a dose-related rise in vitamin E levels in serum, blood platelets, liver and adipose tissue. The higher vitamin E intake was reflected by a twofold increase of vitamin E in serum, platelets and adipose tissue, and a tenfold increase in the liver. The adipose tissue revealed histopathological changes of yellow fat disease, mainly in the low-dose vitamin E group. In the liver microgranulomas of lipofuscin-laden macrophages were seen. Vitamin E was found to decrease but not to prevent the formation of these lesions. The results indicate that protection of marine oils against in vivo oxidation is problematic in the rabbit. It is questionable whether in this animal vitamin E is an adequate biological anti-oxidant for very long chain n-3 fatty acids.
研究了喂食含大量鱼油的半合成日粮的兔子对维生素E的需求。将三组各5只兔子分别喂食含50、100和500毫克/千克维生素E的鱼油日粮。此外，通过使用不同水平的草粉（分别为0.5%、1%和2%）来评估日粮适口性。日粮中添加1%的草粉足以使兔子接受鱼油日粮。维生素E摄入量的增加导致血清、血小板、肝脏和脂肪组织中维生素E水平呈剂量相关上升。较高的维生素E摄入量表现为血清、血小板和脂肪组织中维生素E增加两倍，肝脏中增加十倍。脂肪组织出现了黄脂病的组织病理学变化，主要在低剂量维生素E组。在肝脏中可见充满脂褐素的巨噬细胞形成的微肉芽肿。发现维生素E可减少但不能预防这些病变的形成。结果表明，在兔子体内保护海产油免受氧化存在问题。在这种动物中，维生素E对于极长链n-3脂肪酸是否是一种足够的生物抗氧化剂值得怀疑。
