Chemical Engineering Department, New Mexico State University, Las Cruces, NM 88003, USA.
Bioresour Technol. 2013 Jul;139:308-15. doi: 10.1016/j.biortech.2013.04.045. Epub 2013 Apr 20.
An in situ transesterification approach was demonstrated for converting lipid-rich wet algae (Nannochloropsis salina) into fatty acid ethyl esters (FAEE) under microwave-mediated supercritical ethanol conditions, while preserving the nutrients and other valuable components in the algae. This single-step process can simultaneously and effectively extract the lipids from wet algae and transesterify them into crude biodiesel. Experimental runs were designed to optimize the process parameters and to evaluate their effects on algal biodiesel yield. The algal biomass characterization and algal biodiesel analysis were carried out by using various analytical instruments such as FTIR, SEM-EDS, TLC, GC-MS and transmission electron microscopy (TEM). The thermogravimetric analysis (TGA) under nitrogen and oxygen environments was also performed to examine the thermal and oxidative stability of ethyl esters produced from wet algae. This simple in situ transesterification process using a green solvent and catalyst-free approach can be a potentially efficient route for algal biodiesel production.
采用原位酯交换方法，在微波辅助超临界乙醇条件下，将富含脂质的湿藻（盐生杜氏藻）转化为脂肪酸乙酯（FAEE），同时保留藻类中的营养物质和其他有价值的成分。这种一步法工艺可以同时从湿藻中有效地提取脂质，并将其转化为粗生物柴油。通过设计实验运行来优化工艺参数，并评估其对藻类生物柴油产率的影响。采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜-能谱仪（SEM-EDS）、薄层色谱（TLC）、气相色谱-质谱联用（GC-MS）和透射电子显微镜（TEM）等各种分析仪器对藻生物质进行了表征和对藻生物柴油进行了分析。还进行了氮气和氧气环境下的热重分析（TGA），以检查从湿藻中提取的乙酯的热稳定性和氧化稳定性。这种使用绿色溶剂和无催化剂的简单原位酯交换方法可能是藻类生物柴油生产的一种有效途径。
