Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
Biofouling. 2013;29(5):483-90. doi: 10.1080/08927014.2013.782541. Epub 2013 May 13.
Mussel adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its bulk adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (47 mg l(-1)) of recombinant fp-3 was achieved, and its bulk adhesive strength was measured for the first time; ~0.57 MPa for the unmodified form and ~0.94 and ~2.28 MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the bulk adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (5%). The strong bulk adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadhesive.
贻贝黏附蛋白（MAP）类型 3（fp-3）被认为是贻贝黏附的关键成分之一。然而，由于其数量有限，其整体黏附强度尚未得到表征。在本工作中，实现了重组 fp-3 的可行生产（47 mg l(-1)），并首次测量了其整体黏附强度；未经修饰的形式为0.57 MPa，经 3,4-二羟基-L-苯丙氨酸（DOPA）修饰的形式为0.94 和2.28 MPa，未经和经氧化剂处理的产率分别为 9.6%。此外，还比较了几种 DOPA 修饰的重组 MAP 的整体黏附强度值。经氧化剂处理后的 DOPA 修饰 fp-3 的最大黏附强度强于修饰产率为 6.2%的类型 5（fp-5），且与修饰产率相似（~5%）的杂交类型 fp-131 和 fp-151 相当。重组 fp-3 具有较强的整体黏附性能，表明其作为一种有前途的生物黏附剂具有潜在的应用价值。
