Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China.
Int Immunopharmacol. 2013 Sep;17(1):50-6. doi: 10.1016/j.intimp.2013.04.032. Epub 2013 May 10.
This study investigated the effect of dietary nucleotides (NTs) on immune function in female Balb/C mice, which randomly distributed into six groups: one control group, one NF-free (NF) control group and four NT groups. NTs ranged from 0.0025% to 0.64%. Compared with the control group, the NF could significantly weaken the activity of T lymphocytes and macrophages, as well as decreased the activity of B lymphocytes and NK cell. NF significantly decreased the ratio of CD4(+)/CD8(+), whereas, it increased Tr percentage. In comparison with the NF group, the concentration of serum IL-2 and IL-4 showed an increase trend. Meanwhile, the granular cell macrophages colony stimulating factor (GM-CSF) increased significantly in the 0.04% NT group. The ratio of Th1/Th2 also showed an increasing trend after the supplements of NTs. There were no significant differences between the control and 0.04% NT group. Nevertheless, no significant differences in weight gain and lymphoid organ indices were observed in our study. These results indicate that NT supplements can prevent hypoimmunity which result from NF diet. 0.04% NTs is the healthy optimal supply proportion in mice diet.
本研究探讨了膳食核苷酸（NTs）对雌性 Balb/C 小鼠免疫功能的影响，将其随机分为六组：一组对照组、一组无 NF 对照组（NF）和四组 NT 组。NTs 的浓度范围为 0.0025%至 0.64%。与对照组相比，NF 可显著减弱 T 淋巴细胞和巨噬细胞的活性，同时降低 B 淋巴细胞和 NK 细胞的活性。NF 还显著降低了 CD4(+)/CD8(+)的比例，而 Tr 百分比升高。与 NF 组相比，血清 IL-2 和 IL-4 浓度呈上升趋势。同时，在 0.04%NT 组中，颗粒细胞巨噬细胞集落刺激因子（GM-CSF）显著增加。Th1/Th2 的比例在补充 NTs 后也呈现上升趋势。对照组和 0.04%NT 组之间没有显著差异。然而，本研究中未观察到体重增加和淋巴器官指数的显著差异。这些结果表明，NT 补充剂可以预防 NF 饮食引起的免疫功能低下。0.04%NTs 是小鼠饮食中健康的最佳供应比例。
