School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Sensors (Basel). 2013 May 13;13(5):6204-16. doi: 10.3390/s130506204.
A glassy carbon electrode (GCE) coated with a graphene/polymer film was fabricated for rapid determination of phenols in aqueous solutions. The electrochemical behavior of different phenols at the graphene/polymer-coated GCE was also investigated. In PBS buffer solution with a pH of 6.5, hydroquinone exhibits a well-defined reduction peak at the modified GCE. Based on this, an electrochemical method for the direct determination of phenols is proposed. Investigating different parameters revealed the optimized detection conditions for the electrode are a scan rate of 50 mV/s, dosage of graphene-polyaniline of 8 μL, dosage of tyrosinase of 3 μL, and pH of 6.5. Under the optimal conditions, the reduction peak current varies linearly with the concentration of phenols, with a linear regression equation of I (10(-6)A) = -4.887 × 10(-4)C (mol/L)-5.331 × 10(-6) with a correlation coefficient of 0.9963 and limit of detection (S/N = 3) of 2.00 × 10(-4) mol/L. The electrochemical sensor is also used to detect phenols in actual samples, where it shows great promise for rapid, simple and quantitative detection of phenols.
一种涂有石墨烯/聚合物薄膜的玻碳电极（GCE）被制备用于快速测定水溶液中的酚类物质。还研究了不同酚类物质在石墨烯/聚合物涂层 GCE 上的电化学行为。在 pH 值为 6.5 的 PBS 缓冲溶液中，对苯二酚在修饰后的 GCE 上呈现出良好的还原峰。基于此，提出了一种用于直接测定酚类物质的电化学方法。研究不同参数表明，电极的最佳检测条件为扫描速率为 50 mV/s、石墨烯-聚苯胺用量为 8 μL、酪氨酸酶用量为 3 μL、pH 值为 6.5。在最佳条件下，还原峰电流与酚类物质的浓度呈线性关系，线性回归方程为 I（10^-6 A）=-4.887×10^-4 C（mol/L）-5.331×10^-6，相关系数为 0.9963，检测限（S/N=3）为 2.00×10^-4 mol/L。该电化学传感器还用于检测实际样品中的酚类物质，显示出快速、简单和定量检测酚类物质的巨大潜力。
