Fartas Fuzi Mohamed, Abdullah Jaafar, Yusof Nor Azah, Sulaiman Yusran, Saiman Mohd Izham
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
Sensors (Basel). 2017 May 16;17(5):1132. doi: 10.3390/s17051132.
In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.
在本研究工作中，基于将酪氨酸酶固定在石墨烯修饰的金纳米颗粒/壳聚糖（Gr-Au-Chit/Tyr）纳米复合材料修饰的丝网印刷碳电极（SPCE）上制备了电化学生物传感器，用于检测酚类化合物。通过溶液浇铸法构建纳米复合膜。使用差分脉冲伏安法（DPV）和循环伏安法（CV）研究了所提出的生物传感器对苯酚检测的电催化活性。对pH缓冲液、酶浓度、Gr-Au-Chit比例、富集时间和电位等实验参数进行了优化。该生物传感器对苯酚在0.05至15 μM浓度范围内呈线性，灵敏度为0.624 μA/μM，检测限（LOD）为0.016 μM（S/N = 3）。所提出的传感器还表现出至少一个月的良好重现性、选择性和稳定性。将该生物传感器与高效液相色谱（HPLC）法用于检测实际水样中加标的苯酚进行了比较，结果吻合良好且具有可比性。
