Nahar Z, Sarwar M S, Safiqul Islam M, Rahman A, Nazrul Islam S, Islam M S, Hasnat A
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka, Bangladesh.
Drug Res (Stuttg). 2013 Aug;63(8):424-8. doi: 10.1055/s-0033-1343494. Epub 2013 May 13.
There are sufficient experimental evidences to establish the relationship between the elevated level of malondealdehyde (MDA)-the lipid peroxidation product and depleted level of antioxidants (Vitamin A, E, C and glutathione) in several psychiatric disorders. But previously no study was carried out to determine these components in panic disorder (PD) patients of Bangladesh. This study was conducted to assess the serum concentration of antioxidant vitamins, MDA and glutathione in 54 panic disorder patients and 52 healthy volunteers. Patients were recruited from Bangabandhu Sheikh Mujib Medical University, Bangladesh by random sampling. Serum level of MDA, glutathione and vitamin C were determined by UV spectrophotometric method whereas Vitamins A and E were detected by RP-HPLC method. Data were analyzed by independent t test and Pearson's correlation analysis. It had been found that the PD patients had low level of antioxidants like vitamin A (p=0.041) and vitamin E (p=0.018) than the healthy controls whereas the change of vitamin C is not significant. It had been found that the MDA content was significantly higher (p<0.05) in PD patients than that of controls. There was no significant difference for the glutathione content between the 2 groups. Pearson's correlation coefficient suggested that there were significant negative correlation between the glutathione level and vitamin C (p=0.013) and a positive correlation between the vitamin E and vitamin A (p=0.020) in patient group. Our study reveals that panic disorder patients have considerably higher level of MDA, lower level of antioxidant vitamins and glutathione than the healthy control subjects.
有足够的实验证据证实，在几种精神疾病中，脂质过氧化产物丙二醛（MDA）水平升高与抗氧化剂（维生素A、E、C和谷胱甘肽）水平降低之间存在关联。但此前尚未有研究对孟加拉国惊恐障碍（PD）患者的这些成分进行测定。本研究旨在评估54例惊恐障碍患者和52名健康志愿者血清中抗氧化维生素、MDA和谷胱甘肽的浓度。通过随机抽样从孟加拉国班加班杜·谢赫·穆吉布医科大学招募患者。采用紫外分光光度法测定血清中MDA、谷胱甘肽和维生素C的水平，而采用反相高效液相色谱法检测维生素A和E。数据采用独立t检验和Pearson相关分析进行分析。结果发现，与健康对照组相比，PD患者的维生素A（p=0.041）和维生素E（p=0.018）等抗氧化剂水平较低，而维生素C的变化不显著。结果发现，PD患者的MDA含量显著高于对照组（p<0.05）。两组之间的谷胱甘肽含量无显著差异。Pearson相关系数表明，患者组中谷胱甘肽水平与维生素C之间存在显著负相关（p=0.013），维生素E与维生素A之间存在正相关（p=0.020）。我们的研究表明，惊恐障碍患者的MDA水平明显高于健康对照组，抗氧化维生素和谷胱甘肽水平则低于健康对照组。
