Islam M R, Ahmed M U, Islam M S, Sayeed M S B, Sadia F, Chowdhury Z S, Nahar Z, Hasnat A
Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh.
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali, Bangladesh.
Drug Res (Stuttg). 2014 Aug;64(8):406-11. doi: 10.1055/s-0033-1358758. Epub 2013 Nov 27.
The relationship between the elevated levels of serum malondialdehyde, depleted level of antioxidants (vitamin A, E and C) and altered level of immunoglobulins (IgA, IgG and IgM) in several psychiatric disorders has been established by various experimental evidences over the past few years. But previously no study was carried out to determine these components in patients suffering from generalized anxiety disorder (GAD) in Bangladesh. This study was conducted to compare the serum concentration of these components in GAD patients and healthy volunteers; matched by socioeconomic and sociodemographic parameters. Serum level of malondialdehyde and vitamin C were determined by UV spectrophotometric method, vitamins A and E were detected by RP-HPLC method whereas immunoglobulin levels were determined by turbidimetric method. Data were analyzed by independent t-test, Pearson's correlation and regression analysis. Significantly lower level of vitamin E (p<0.05) and significantly higher level of vitamin C were found in GAD patients than the healthy controls, whereas the change of vitamin A was insignificant. Serum malondialdehyde content was significantly higher (p<0.05) and IgM level was significantly (p<0.05) higher than that of the controls. Change in concentrations of IgG and IgA were insignificant (p>0.05). Pearson's correlation coefficient suggested that there were some significant positive and negative correlations among these tested components. Our study reveals that GAD patients have considerably higher level of malondialdehyde, immunoglobulins and altered level of antioxidant vitamins. These findings may play a key role in the diagnosis and treatment of GAD patients.
过去几年，各种实验证据证实了几种精神疾病中血清丙二醛水平升高、抗氧化剂（维生素A、E和C）水平降低以及免疫球蛋白（IgA、IgG和IgM）水平改变之间的关系。但此前在孟加拉国，尚未开展过针对广泛性焦虑症（GAD）患者这些成分的研究。本研究旨在比较GAD患者和健康志愿者中这些成分的血清浓度；两组在社会经济和社会人口统计学参数方面相匹配。采用紫外分光光度法测定血清丙二醛和维生素C水平，采用反相高效液相色谱法检测维生素A和E，采用比浊法测定免疫球蛋白水平。通过独立样本t检验、Pearson相关性分析和回归分析对数据进行分析。结果发现，GAD患者的维生素E水平显著低于健康对照组（p<0.05），维生素C水平显著高于健康对照组，而维生素A的变化不显著。GAD患者的血清丙二醛含量显著高于对照组（p<0.05），IgM水平也显著高于对照组（p<0.05）。IgG和IgA浓度的变化不显著（p>0.05）。Pearson相关系数表明，这些检测成分之间存在一些显著的正相关和负相关。我们的研究表明，GAD患者的丙二醛、免疫球蛋白水平显著升高，抗氧化维生素水平改变。这些发现可能在GAD患者的诊断和治疗中发挥关键作用。
