Kirkwood Jay S, Maier Claudia, Stevens Jan F
Linus Pauling Institute and Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon, USA.
Curr Protoc Toxicol. 2013 May;Chapter 4:Unit4.39. doi: 10.1002/0471140856.tx0439s56.
At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating this task. Performing multiple sample extractions, concentrating sample extracts, and using several separation and detection methods are common strategies to overcome these challenges but require a great amount of resources. This protocol describes the untargeted, metabolic profiling of polar and nonpolar metabolites with a single extraction and using a single analytical platform.
在其最具雄心的层面上，非靶向代谢组学旨在对给定系统中的所有代谢物进行表征和定量。代谢物的浓度范围通常很广，且具有多样的物理性质，这使得这项任务变得复杂。进行多次样品提取、浓缩样品提取物以及使用多种分离和检测方法是克服这些挑战的常见策略，但需要大量资源。本方案描述了通过单次提取并使用单一分析平台对极性和非极性代谢物进行非靶向代谢谱分析的方法。
