Raabe O, Shell K, Goessl A, Crispens C, Delhasse Y, Eva A, Scheiner-Bobis G, Wenisch S, Arnhold S
Institute of Veterinary -Anatomy, -Histology, and -Embryology, Justus-Liebig University of Giessen Germany.
Am J Stem Cells. 2013 Mar 8;2(1):62-73. Print 2013.
Mesenchymal stem cells are regarded as common cellular precursors of the musculoskeletal tissue and are responsible for tissue regeneration in the course of musculoskeletal disorders. In equine veterinary medicine extracorporeal shock wave therapy (ESWT) is used to optimize healing processes of bone, tendon and cartilage. Nevertheless, little is known about the effects of the shock waves on cells and tissues. Thus, the aim of this study was to investigate the influence of focused ESWT on the viability, proliferation, and differentiation capacity of adipose tissue-derived mesenchymal stem cells (ASCs) and to explore its effects on gap junctional communication and the activation of signalling cascades associated with cell proliferation and differentiation. ASCs were treated with different pulses of focused ESWT. Treated cells showed increased proliferation and expression of Cx43, as detected by means of qRT-PCR, histological staining, immunocytochemistry and western blot. At the same time, cells responded to ESWT by significant activation (phosphorylation) of Erk1/2, detected in western blots. No significant effects on the differentiation potential of the ASCs were evident. Taken together, the present results show significant effects of shock waves on stem cells in vitro.
间充质干细胞被视为肌肉骨骼组织常见的细胞前体，在肌肉骨骼疾病过程中负责组织再生。在马兽医医学中，体外冲击波疗法（ESWT）用于优化骨骼、肌腱和软骨的愈合过程。然而，关于冲击波对细胞和组织的影响知之甚少。因此，本研究的目的是研究聚焦ESWT对脂肪组织来源的间充质干细胞（ASC）的活力、增殖和分化能力的影响，并探讨其对缝隙连接通讯以及与细胞增殖和分化相关的信号级联激活的影响。用不同脉冲的聚焦ESWT处理ASC。通过qRT-PCR、组织学染色、免疫细胞化学和蛋白质印迹检测，处理后的细胞显示增殖增加和Cx43表达增加。同时，在蛋白质印迹中检测到细胞通过Erk1/2的显著激活（磷酸化）对ESWT作出反应。对ASC的分化潜能没有明显影响。综上所述，目前的结果表明冲击波在体外对干细胞有显著影响。
