Wang Wei, Yang Yan, Zheng Xiao-Dong, Huang Shu-Qiong, Guo Lei, Kong Jian-Qiang, Cheng Ke-Di
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines & Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Yao Xue Xue Bao. 2013 Feb;48(2):187-92.
The synthetic biology matures to promote the heterologous biosynthesis of the well-known drug paclitaxel that is one of the most important and active chemotherapeutic agents for the first-line clinical treatment of cancer. This review focuses on the construction and regulation of the biosynthetic pathway of paclitaxel intermediates in both Escherichia coli and Saccharomyces cerevisiae. In particular, the review also features the early efforts to design and overproduce taxadiene and the bottleneck of scale fermentation for producing the intermediates.
合成生物学逐渐成熟，以促进著名药物紫杉醇的异源生物合成，紫杉醇是癌症一线临床治疗中最重要且最有效的化疗药物之一。本综述重点关注大肠杆菌和酿酒酵母中紫杉醇中间体生物合成途径的构建与调控。特别地，该综述还介绍了设计并过量生产紫杉二烯的早期努力以及生产中间体的规模发酵瓶颈。
