Department of Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo, New York, USA.
Appl Environ Microbiol. 2012 Apr;78(8):2497-504. doi: 10.1128/AEM.07391-11. Epub 2012 Jan 27.
Escherichia coli offers unparalleled engineering capacity in the context of heterologous natural product biosynthesis. However, as with other heterologous hosts, cellular metabolism must be designed or redesigned to support final compound formation. This task is at once complicated and aided by the fact that the cell does not natively produce an abundance of natural products. As a result, the metabolic engineer avoids complicated interactions with native pathways closely associated with the outcome of interest, but this convenience is tempered by the need to implement the required metabolism to allow functional biosynthesis. This review focuses on engineering E. coli for the purpose of polyisoprene formation, as it is related to isoprenoid compounds currently being pursued through a heterologous approach. In particular, the review features the compound paclitaxel and early efforts to design and overproduce intermediates through E. coli.
大肠杆菌在异源天然产物生物合成方面提供了无与伦比的工程能力。然而，与其他异源宿主一样，细胞代谢必须进行设计或重新设计，以支持最终化合物的形成。这项任务既复杂又复杂，因为细胞本身并不大量产生天然产物。因此，代谢工程师避免了与目标结果密切相关的天然途径的复杂相互作用，但这种便利性受到需要实施所需代谢以允许功能生物合成的限制。本文综述了为聚异戊二烯形成而对大肠杆菌进行工程改造的情况，因为它与目前通过异源方法研究的异戊二烯化合物有关。特别是，该综述介绍了化合物紫杉醇和通过大肠杆菌设计和过量生产中间体的早期努力。
