[高效液相色谱-二极管阵列检测法同时测定柘树及构树根茎中4种黄酮类成分]

Li Bo, Wang Mei, Tan Ya-Nan, Tong Miao-Miao, Zhai Yan-Jun

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.

Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(2):167-70.

OBJECTIVE

To establish a HPLC-DAD method for the determination of axifolin, naringenin, quercetin and kaempferol in Cudrania tricuspidata and C. cochinchinensis in order to provide a scientific reference for species identification and quality evaluation, by establishing.

METHOD

The determination was performed by HPLC-DAD on an Agilent C18 column (4.6 mm x 150 mm, 5 microm) by gradient elution (0-15 min, 35%-50% A; 15-30 min, 50% - 65% A) using methanol (A) and 0.1% phosphoric acid (B) as the mobile phase. The flow rate was 1 mL x min(-1). The detection wavelength was 290 nm for taxifolin and naringenin, 365 nm for quercetin and kaempferol with column temperature at 30 degrees C.

RESULT

The content of axifolin and quercetin in the root of C. tricuspidata were remarkably higher than that in the root of C. cochinchinensis, and the content in stem of C. tricuspidata was also higher than that in the stem of C. cochinchinensis, the content of axifolin and quercetin was variable in different species. The content of naringenin and kaempferol in the root of C. cochinchinensis was visibly higher than that in the root of C. tricuspidata, and the content in the stems of the two herbs was similar, the content of naringenin and kaempferol was visibly variable in different medicinal parts of the herb, but similar between the two herbs.

CONCLUSION

There's some difference of the content of the four ingredients in different medicinal parts and different herbs, so clinical use should not be confused.

目的

建立高效液相色谱 - 二极管阵列检测法（HPLC - DAD）测定柘树和构树中落新妇苷、柚皮素、槲皮素和山柰酚的含量，以便为物种鉴定和质量评价提供科学参考。

方法

采用HPLC - DAD法，在安捷伦C18柱（4.6 mm×150 mm，5μm）上进行梯度洗脱（0 - 15分钟，35% - 50%A；15 - 30分钟，50% - 65%A），以甲醇（A）和0.1%磷酸（B）为流动相。流速为1 mL·min⁻¹。落新妇苷和柚皮素的检测波长为290 nm，槲皮素和山柰酚的检测波长为365 nm，柱温为30℃。