链霉菌属一种菌株中胆固醇氧化酶的产生、纯化及某些特性
Production, purification and some properties of cholesterol oxidase from a Streptomyces Sp.
作者信息
Lartillot S, Kedziora P
机构信息
Department of Applied Biology, Institut Universitaire de Technologie, Dijon, France.
出版信息
Prep Biochem. 1990;20(1):51-62. doi: 10.1080/00327489008050176.
A Streptomyces Sp strain producing appreciable levels of cholesterol oxidase was selected. In the culture medium, cholesterol oxidase activity can reach 13,000 UI/l. Cholesterol oxidase was purified by means of centrifugation, tangential ultrafiltration, ion-exchange chromatography and gel filtration with a yield of 42% to a specific activity of 54 UI/mg. Its molecular weight determined by SDS-PAGE was 55,000. Its optimum activity pH is about 7.
筛选出了一株能产生可观水平胆固醇氧化酶的链霉菌菌株。在培养基中，胆固醇氧化酶活性可达13000 UI/l。通过离心、切向超滤、离子交换色谱和凝胶过滤对胆固醇氧化酶进行纯化，得率为42%，比活性为54 UI/mg。通过SDS-PAGE测定其分子量为55000。其最适活性pH约为7。
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