使用荧光标记转基因小鼠对早期小鼠胚胎进行活体成像。

Live imaging of early mouse embryos using fluorescently labeled transgenic mice.

作者信息

Abe Takaya, Aizawa Shinichi, Fujimori Toshihiko

机构信息

Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology (CDB), Kobe, Japan.

出版信息

Methods Mol Biol. 2013;1052:101-8. doi: 10.1007/7651_2013_20.

DOI:10.1007/7651_2013_20

PMID:23674079

Abstract

Live imaging is a powerful approach to understanding the dynamic processes that occur during development. Periimplantation mouse embryos are transparent, making them suitable for live imaging. In this chapter we describe the culturing and live imaging of mouse embryos, and also introduce the reporter mouse lines for organelle labeling we recently established.

摘要

实时成像技术是一种了解发育过程中动态变化的有效方法。植入前的小鼠胚胎是透明的，这使其适合进行实时成像。在本章中，我们将描述小鼠胚胎的培养和实时成像，并介绍我们最近建立的用于细胞器标记的报告基因小鼠品系。