Grote David, Marcotte Michael, Bouchard Maxime
Goodman Cancer Research Centre and Department of Biochemistry, McGill University, Montreal, QC, Canada.
Methods Mol Biol. 2012;886:31-43. doi: 10.1007/978-1-61779-851-1_4.
Embryonic development is a highly dynamic process involving complex tissue interactions and movements. Recent progress in cell labeling, image acquisition, and image processing technologies has brought the study of embryo morphogenesis to another level. It is now possible to visualize in real time the dynamic morphogenetic changes occurring in vivo and to reconstitute and quantify them in 4D rendering. However, extended live embryo imaging remains challenging in terms of embryo survival and minimization of phototoxicity. Here, we describe a procedure to image the developing mesonephros for up to 16 h in intact mouse embryos. This method can easily be adapted to the imaging of other structures at similar developmental stages.
胚胎发育是一个高度动态的过程，涉及复杂的组织相互作用和运动。细胞标记、图像采集和图像处理技术的最新进展将胚胎形态发生的研究提升到了一个新的水平。现在可以实时可视化体内发生的动态形态发生变化，并通过4D渲染对其进行重建和量化。然而，延长活胚胎成像在胚胎存活和将光毒性降至最低方面仍然具有挑战性。在这里，我们描述了一种在完整小鼠胚胎中对发育中的中肾进行长达16小时成像的方法。该方法可以很容易地适用于对类似发育阶段的其他结构进行成像。
