Meissner H O, Mrozikiewicz P, Bobkiewicz-Kozlowska T, Mscisz A, Kedzia B, Lowicka A, Reich-Bilinska H, Kapczynski W, Barchia I
Faculty of Health Studies, Charles Sturt University & Therapeutic Research International, GPO Box 4792, Sydney 2001, Australia;
Int J Biomed Sci. 2006 Sep;2(3):260-72.
Ovariectomized rats were used in a model laboratory study to examine biochemical and pharmacodynamic effects of pre-gelatinized organic preparation of Lepidium peruvianum Chacon (Maca-GO). Biochemical and Pharmacodynamic effects of Maca-GO (250 mg Maca-GO per kg body weight (bw) administered by intubation twice daily) were assessed in a 28 day model laboratory study on ovariectomized (by laparoscopy) Wistar rats with pharmacodynamic tests performed at the conclusion of the trial followed by blood collection for morphology and biochemical tests. Toxicity of Maca-GO used in the study was determined in bioassay on mice and rats. Anti-depressive function (Porsolt's test) and anxiolytic sedative and cognitive effects (using elevated-plus maze, locomotor activity and passive avoidance tests) were assessed against control (laparotomized female rats with intact ovaries). In addition to blood morphology, the following blood serum constituents were analyzed: Estrogen (E2), Progesterone (PGS), Cortisol (CT), Adrenocorticotropic Hormone (ACTH), Thyroid Hormones (TSH, T3, and T4), Iron (Fe) and lipid profile (Triglycerides, Total Cholesterol, LDL, HDL). Analytically-determined non-toxic status of Maca-GO was confirmed in bioassays when applied to mice and rats at levels of 0.5 and up to 15mg/kg bw which shows it safe use in humans with the LD50>15 mg/kg bw. Maca-GO showed a distinctive, (P<0.05) antidepressant-like and sedative effect in ovariectomized rats only, while there was no anxiolytic activity nor disturbance of cognitive function observed in both, test and control animals. Observed in this study balancing effect of Maca-GO on sex hormone levels show its potential as a safe preparation for use in correcting physiological symptoms characteristic in postmenopausal stage with an indication of potentially even more value for its use in pre-menopausal women.
在一项模型实验室研究中，使用去卵巢大鼠来检验秘鲁玛卡（Maca-GO）预糊化有机制剂的生化和药效学作用。在一项为期28天的模型实验室研究中，对通过腹腔镜去卵巢的Wistar大鼠评估了Maca-GO（每天经插管给药两次，每千克体重250毫克Maca-GO）的生化和药效学作用，在试验结束时进行药效学测试，随后采集血液进行形态学和生化测试。研究中使用的Maca-GO的毒性在小鼠和大鼠的生物测定中确定。针对对照组（具有完整卵巢的剖腹雌性大鼠）评估了抗抑郁功能（波索尔特试验）以及抗焦虑镇静和认知作用（使用高架十字迷宫、运动活动和被动回避试验）。除了血液形态学外，还分析了以下血清成分：雌激素（E2）、孕酮（PGS）、皮质醇（CT）、促肾上腺皮质激素（ACTH）、甲状腺激素（TSH、T3和T4）、铁（Fe）和血脂谱（甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白）。当以0.5至高达15毫克/千克体重的剂量应用于小鼠和大鼠时，生物测定证实了分析确定的Maca-GO无毒状态，这表明其在人类中的安全使用，半数致死剂量>15毫克/千克体重。Maca-GO仅在去卵巢大鼠中显示出显著的（P<0.05）抗抑郁样和镇静作用，而在试验组和对照组动物中均未观察到抗焦虑活性或认知功能障碍。本研究中观察到的Maca-GO对性激素水平的平衡作用表明，它有可能作为一种安全制剂，用于纠正绝经后阶段的生理症状，甚至对绝经前女性可能更有价值。
