Bousette Nicolas, Chouiali Fazila, Ohlstein Eliot H, Douglas Stephen A, Giaid Adel
Division of Cardiology, Montreal General Hospital, McGill University Health Center, Montreal, Quebec, Canada;
Int J Biomed Sci. 2007 Mar;3(1):38-45.
Treatment for symptomatic atherosclerosis is being carried out by balloon mediated angioplasty, with or without stent implantation, more and more frequently. Although advances with the development of drug eluting stents have improved prognosis, restenosis is still the most limiting factor for this treatment modality. Urotensin-II (UII), a small pleiotropic vasoactive peptide is increasingly being recognized as a contributory factor in cardiovascular diseases. We qualitatively evaluated UII immunoreactivity (IR) in three models of balloon angioplasty mediated restenosis. Specifically, we performed balloon angioplasty in the ilio-femoral arteries of New Zealand White Rabbits (NZWR) fed either a normal chow or high fat diet. In addition, UIIIR was also assessed in stent implanted abdominal aortae of NZWR fed a high fat diet. UII was constitutively expressed in the endothelium of all arterial segments evaluated. Abundant expression of UII was associated with lesion progression, particularly in myointimal cells, and less so in medial smooth muscle cells (SMC). The strongest UII-IR was observed in foam cells of animals fed a high fat diet. We demonstrate abundant expression of UII in regenerating endothelial cells and myointimal cells in vascular lesions following balloon mediated angioplasty and stent implantation in both animals fed a normal chow and high fat diet.
有症状动脉粥样硬化的治疗越来越多地通过球囊介导的血管成形术进行，无论是否植入支架。尽管随着药物洗脱支架的发展取得了进展，改善了预后，但再狭窄仍然是这种治疗方式的最限制因素。尾加压素-II（UII）是一种具有多种作用的小血管活性肽，越来越被认为是心血管疾病的一个促成因素。我们对球囊血管成形术介导的再狭窄的三种模型中的UII免疫反应性（IR）进行了定性评估。具体而言，我们在喂食正常饲料或高脂饮食的新西兰白兔（NZWR）的髂股动脉中进行了球囊血管成形术。此外，还评估了喂食高脂饮食的NZWR植入支架的腹主动脉中的UIIIR。UII在所有评估的动脉段内皮中组成性表达。UII的丰富表达与病变进展相关，特别是在肌内膜细胞中，在内膜平滑肌细胞（SMC）中较少。在喂食高脂饮食的动物的泡沫细胞中观察到最强的UII-IR。我们证明，在喂食正常饲料和高脂饮食的动物中，球囊介导的血管成形术和支架植入后，血管病变中的再生内皮细胞和肌内膜细胞中UII表达丰富。
