Al-Arfaj Nawal Ahmad, Al-Abdulkareem Eman Abdullah, Aly Fatma Ahmad
Department of Chemistry, College of Science, Women Student-Medical Studies and Science Sections, King Saud University, Saudi Arabia.
Int J Biomed Sci. 2008 Dec;4(4):310-8.
Square-wave adsorptive cathodic stripping voltammetry was used to determine pioglitazone HCl in Britton Robinson buffer of pH5. The adsorptive cathodic peak was observed at -1.5 V vs. Ag/AgCl. The peak response was characterized with respect to pH, supporting electrolyte, frequency, scan increment, pulse-amplitude, accumulation potential and pre-concentration time. Under optimal conditions, the peak current is proportional to the concentration of pioglitazone HCl, and a linear calibration graphs were obtained within the concentration levels of 10(-8) and 10(-4) M following different accumulation time periods (0-300 s). The obtained results were analyzed and the statistical parameters were calculated. The detection limit is 8.08 × 10(-9) M (3.17 ng ml(-1)) using 300 s pre-concentration time, whereas the quantitative limit is 2.45 × 10(-8) M (9.63 ng ml(-1)). The proposed method was applied to assay the drug in pharmaceutical formulations and biological fluids. The pharmacokinetic parameters of drug in human plasma were estimated as: C max=785.8 ng ml(-1), t max=1.5 h, K e=0.125 h(-1) and t 1/2=8 h which are favorably compared with those reported in literature.
采用方波吸附阴极溶出伏安法在pH5的 Britton Robinson缓冲溶液中测定盐酸吡格列酮。相对于Ag/AgCl参比电极，在-1.5 V处观察到吸附阴极峰。对峰响应在pH、支持电解质、频率、扫描增量、脉冲幅度、富集电位和预富集时间等方面进行了表征。在最佳条件下，峰电流与盐酸吡格列酮的浓度成正比，在不同预富集时间段（0 - 300 s）下，在10(-8)至10(-4) M的浓度范围内获得了线性校准曲线。对所得结果进行了分析并计算了统计参数。使用300 s预富集时间时，检测限为8.08×10(-9) M（3.17 ng ml(-1)），定量限为2.45×10(-8) M（9.63 ng ml(-1)）。所提出的方法应用于药物制剂和生物流体中药物的测定。人体血浆中药物的药代动力学参数估计为：C max = 785.8 ng ml(-1)，t max = 1.5 h，K e = 0.125 h(-1)，t 1/2 = 8 h，与文献报道的结果相比具有优势。
