Song Yan, Li Na, He Liu, Xun Tang, Chen Dafang, Hu Yonghua
Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China; Key Laboratory of Epidemiology, Ministry of Education, Beijing, China.
Int J Biomed Sci. 2009 Sep;5(3):305-12.
Upstream transcription factor 1 (USF1) is capable of controlling various members in glucose and lipid metabolism pathways. Much evidence suggests that the rs3737787 polymorphism in the USF1 gene may lead to alteration of lipid metabolism. The objective of this study was to test the association between rs3737787 and type 2 diabetes mellitus (T2DM) and its related lipid metabolic traits in the Chinese population. A total of 287 eligible T2DM families were chosen in Beijing. A set of questionnaires was administered to obtain information on demographic characteristics. Physical measurements were recorded. DNA was extracted from blood samples and genotyped using PCR-RFLP method. Statistical analyses including linkage analysis and family-based association test were performed to detect the relationship between rs3737787 and T2DM related traits. In the non-parametric linkage analysis, it was observed that rs3737787 is potentially linked with triglyceride and apolipoprotein E levels, where the logarithm of the odds scores were 0.87 (p=0.02) and 1.96 (p=0.001), respectively. Similar results were obtained in the multi-factorial generalized estimating equation analysis. Using different statistical approaches, in this study, we have confirmed that the single nucleotide polymorphism rs3737787 is related to triglyceride and apolipoprotein E levels in type 2 diabetes mellitus families.
上游转录因子1（USF1）能够调控葡萄糖和脂质代谢途径中的多种成分。大量证据表明，USF1基因中的rs3737787多态性可能导致脂质代谢改变。本研究的目的是检验rs3737787与中国人群2型糖尿病（T2DM）及其相关脂质代谢特征之间的关联。在北京共选取了287个符合条件的T2DM家庭。发放了一套问卷以获取人口统计学特征信息。记录了体格测量数据。从血样中提取DNA，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。进行了包括连锁分析和基于家系的关联检验在内的统计分析，以检测rs3737787与T2DM相关特征之间的关系。在非参数连锁分析中，观察到rs3737787与甘油三酯和载脂蛋白E水平可能存在连锁关系，其中优势对数得分分别为0.87（p = 0.02）和1.96（p = 0.001）。在多因素广义估计方程分析中也得到了类似结果。在本研究中，我们使用不同的统计方法证实了单核苷酸多态性rs3737787与2型糖尿病家庭中的甘油三酯和载脂蛋白E水平相关。
