El-Enany Nahed, El-Sherbiny Dina, Belal Fathalla
Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt;
Int J Biomed Sci. 2010 Jun;6(2):128-34.
The voltammetric behaviour of cyproterone acetate (CPA) was studied using direct current (DCt) and differential pulse polarography (DPP). The drug manifests cathodic waves over the pH range of 4-11.8. In Britton-Robinson buffer (BRb) of pH 10, the diffusion current-concentration relationship was found to be rectilinear over the range 3.2-32 μg/mL and 0.5-14 μg/mL using DCt and DPP modes, respectively, with minimum limits of detection (LOD) of 0.13 μg/mL using the DDP. The diffusion-current constant (Id) was 9.29 ± 0.046 (n=9). The proposed method was successfully applied to the determination of the studied compound in its formulations. The mean percentage recoveries in tablets were 99.48 ± 1.25 and 100.01 ± 1.07 (n=4) using DCt and DPP modes, respectively. The results obtained were in agreement with those of the reference method. A proposal for the electrode reaction was postulated.
采用直流极谱法（DCt）和微分脉冲极谱法（DPP）研究了醋酸环丙孕酮（CPA）的伏安行为。该药物在pH值4 - 11.8范围内呈现阴极波。在pH值为10的 Britton - Robinson缓冲液（BRb）中，使用DCt模式时，在3.2 - 32μg/mL范围内扩散电流与浓度关系呈线性，使用DPP模式时，在0.5 - 14μg/mL范围内呈线性，使用DDP时最低检测限（LOD）为0.13μg/mL。扩散电流常数（Id）为9.29 ± 0.046（n = 9）。所提出的方法成功应用于其制剂中该研究化合物的测定。使用DCt和DPP模式时，片剂中的平均回收率分别为99.48 ± 1.25和100.01 ± 1.07（n = 4）。所得结果与参考方法的结果一致。推测了电极反应。
