Walash M I, Belal F, El-Enany N, Elmansi H
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.
Int J Biomed Sci. 2010 Dec;6(4):327-34.
Spectrophotometric and spectrofluorimetric methods were developed for the determination of dothiepin hydrochloride (DOP) in different dosage forms. The spectrophotometric method (Method I) is based on formation of a binary complex with eosin at 540 nm in acetate buffer of pH3.7. The absorbance-concentration plot is rectilinear over the range 1-10 μg/mL with LOD of 0.18 μg/mL and LOQ of 0.54 μg/mL. The spectroflurimetric method (Method II) is based on the quantitative quenching effect of Dothiepin on the native fluorescence of eosin at the same pH. The quenching of the fluorescence of eosin was measured at 543 nm after excitation at 304 nm. The fluorescence-concentration plot is rectilinear over the range 0.3-8 μg/ mL with LOD of 0.11 μg/mL and LOQ of 0.34 μg/mL. The proposed methods were successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods respectively.
开发了分光光度法和荧光分光光度法用于测定不同剂型中的盐酸多塞平（DOP）。分光光度法（方法I）基于在pH3.7的醋酸盐缓冲液中与曙红形成二元络合物，在540nm处进行测定。吸光度-浓度曲线在1-10μg/mL范围内呈线性，检测限为0.18μg/mL，定量限为0.54μg/mL。荧光分光光度法（方法II）基于在相同pH下多塞平对曙红天然荧光的定量猝灭作用。在304nm激发后，于543nm处测量曙红荧光的猝灭。荧光-浓度曲线在0.3-8μg/mL范围内呈线性，检测限为0.11μg/mL，定量限为0.34μg/mL。所提出的方法成功应用于含该药物的市售片剂和胶囊的分析。将结果与参考方法进行统计比较，结果显示良好的一致性，证明两种方法在准确度和精密度上分别无显著差异。
