Reply to Huang et al.: Slow proton exchange can duplicate the number of species observed in single-molecule experiments of protein folding.
作者信息
Campos Luis A, Muñoz Victor
出版信息
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1242-3. doi: 10.1073/pnas.1219166110.
Abstract
摘要
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Reply to Huang et al.: Slow proton exchange can duplicate the number of species observed in single-molecule experiments of protein folding.回复黄等人：缓慢的质子交换可以重现蛋白质折叠单分子实验中观察到的物种数量。
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1242-3. doi: 10.1073/pnas.1219166110.
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Reply to Campos and Muñoz: Why phosphate is a bad buffer for guanidinium chloride titrations.对坎波斯和穆尼奥斯的回复：为何磷酸盐不适用于氯化胍滴定的缓冲剂。
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1244-5. doi: 10.1073/pnas.1303286110.
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Don't waste good methods on bad buffers and ambiguous data.不要把好方法浪费在糟糕的缓冲液和模糊的数据上。
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Slow proton transfer coupled to unfolding explains the puzzling results of single-molecule experiments on BBL, a paradigmatic downhill folding protein.与解折叠耦合的慢质子转移解释了对BBL（一种典型的下坡折叠蛋白）进行单分子实验所得出的令人困惑的结果。
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Reply to Campos and Muñoz: Why phosphate is a bad buffer for guanidinium chloride titrations.对坎波斯和穆尼奥斯的回复：为何磷酸盐不适用于氯化胍滴定的缓冲剂。
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1244-5. doi: 10.1073/pnas.1303286110.
本文引用的文献
1
Don't waste good methods on bad buffers and ambiguous data.不要把好方法浪费在糟糕的缓冲液和模糊的数据上。
Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):E331-2. doi: 10.1073/pnas.1217840110. Epub 2013 Jan 17.
2
Exploring one-state downhill protein folding in single molecules.在单分子中探索单态下坡蛋白折叠。
Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):179-84. doi: 10.1073/pnas.1111164109. Epub 2011 Dec 19.
3
Carboxyl pK(a) values and acid denaturation of BBL.Carboxyl pK(a) values and acid denaturation of BBL.
J Mol Biol. 2010 Oct 22;403(2):313-27. doi: 10.1016/j.jmb.2010.08.052. Epub 2010 Sep 15.
4
Direct observation of barrier-limited folding of BBL by single-molecule fluorescence resonance energy transfer.通过单分子荧光共振能量转移直接观察BBL的屏障限制折叠。
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16239-44. doi: 10.1073/pnas.0909126106. Epub 2009 Sep 11.
5
pH corrections and protein ionization in water/guanidinium chloride.水/氯化胍中的pH校正与蛋白质电离
Biophys J. 2001 Dec;81(6):3489-502. doi: 10.1016/S0006-3495(01)75980-2.
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