Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, 98 Fenghuang Road, Zunyi, 563002, Guizhou Province, China.
J Mater Sci Mater Med. 2013 Aug;24(8):2037-46. doi: 10.1007/s10856-013-4944-z. Epub 2013 May 16.
Bone marrow-derived mesenchymal stem cells (BMSCs) were seeded in a three-dimensional scaffold of silk fibroin (SF) and chitosan (CS) to repair cartilage defects in the rabbit knee. Totally 54 rabbits were randomly assigned to BMSCs + SF/CS scaffold, SF/CS scaffold and control groups. A cylindrical defect was created at the patellofemoral facet of the right knee of each rabbit and repaired by scaffold respectively. Samples were prepared at 4, 8 and 12 weeks post-surgery for gross observation, hematoxylin-eosin and toluidine blue staining, type II collagen immunohistochemistry, Wakitani histology. The results showed that differentiated BMSCs proliferated well in the scaffold. In the BMSCs + SF/CS scaffold group, the bone defect was nearly repaired, the scaffold was absorbed and immunohistochemistry was positive. In the SF/CS scaffold alone group, fiber-like tissues were observed, the scaffold was nearly degraded and immunohistochemistry was weakly positive. In the control group, the defect was not well repaired and positive immunoreactions were not detected. Modified Wakitani scores were superior in the BMSCs + SF/CS scaffold group compared with those in other groups at 4, 8 and 12 weeks (P < 0.05). A SF/CS scaffold can serve as carrier for stem cells to repair cartilage defects and may be used for cartilage tissue engineering.
骨髓间充质干细胞（BMSCs）接种于丝素纤维（SF）和壳聚糖（CS）的三维支架中，以修复兔膝关节软骨缺损。总共 54 只兔子被随机分配到 BMSCs+SF/CS 支架、SF/CS 支架和对照组。在每个兔子的髌股关节面处创建一个圆柱形缺损，并分别用支架修复。术后 4、8 和 12 周分别采集标本进行大体观察、苏木精-伊红和甲苯胺蓝染色、II 型胶原免疫组化、Wakitani 组织学检查。结果显示，分化的 BMSCs 在支架中增殖良好。在 BMSCs+SF/CS 支架组中，骨缺损几乎得到修复，支架被吸收，免疫组化呈阳性。在单独的 SF/CS 支架组中，观察到纤维状组织，支架几乎降解，免疫组化呈弱阳性。在对照组中，缺损未得到良好修复，未检测到阳性免疫反应。在 4、8 和 12 周时，BMSCs+SF/CS 支架组的改良 Wakitani 评分优于其他组（P<0.05）。SF/CS 支架可作为干细胞的载体修复软骨缺损，可用于软骨组织工程。
